0000000000204669
AUTHOR
Varis Karitans
Phase retrieval of vitreous floaters: simulation experiment
Knowledge of the structure of vitreous floaters is crucial to evaluate the need for surgical removal of these floaters. We simulated the phase retrieval of microstructures simulating vitreous floaters by an algorithm PhaseLift and investigate the effects of various parameters on the retrieved phase. The object under test was modulated and the coded diffraction patterns were calculated. Next, PhaseLift was used to retrieve the phase. In the current study, we simulate the effect of Gaussian and Poison noise on the phase retrieval of pure phase objects. We apply an iterative algorithm PhaseLift for phase retrieval as this algorithm requires a very few modulating masks and is able to retrieve t…
Phase retrieval of a Kolmogorov phase screen from very sparse data using four binary masks
We investigate experimentally the phase retrieval of a Kolmogorov phase screen from very sparse data by modulating its amplitude with four binary masks and compare the retrieved phase screen to the ground truth measured with a surface profiler. Previously, we have shown in simulations that this kind of modulation can be successfully used for the phase retrieval of a Kolmogorov phase screen. After subtracting the ground truth from the retrieved phase screen, the root-mean-square error decreased from 0.14 µm to 0.10 µm. We conclude that a Kolmogorov phase screen can be recovered using simple modulation and very sparse data.
Validation of a method for measuring the retinal thickness with Shack–Hartmann aberrometry in an artificial eye
In Shack–Hartmann aberrometry, it is assumed that a wave front emerges from a single point focused on a retina. However, the retina is a multi-layered structure and reflections may occur from several layers. This may result in several overlapping spot patterns on the CCD due to different vergences of the outgoing wave fronts. The amount by which these spot patterns are displaced may contain information about the retinal thickness. In this study, we perform simulations of formation of double spots in a living eye and also apply this method to measure the thickness of an artificial retina with a simple structure. We also compare the results obtained with artificial eye and compare them to the…
Dependence of Perceived Purity of a Chromatic Stimulus on Saturation Adaptation
Background and Objective. The purpose of sensory adaptation of the visual system is to adjust sensitivity of the photoreceptors to optimize the dynamic range of response of the visual system. It has been shown in numerous research papers that chromatic adaptation influences both color appearance and color discrimination. However, there are almost no studies in which the influence of chromatic adaptation on perceived purity has been investigated. Therefore, the aim of this study was to investigate how chromatic adaptation to stimuli with certain saturation influences perceived purity of test stimuli with the same hue but different saturation. Material and Methods. As the stimuli were modulat…
Optical phase retrieval using four rotated versions of a single binary mask – simulation results
In signal processing one often faces the phase problem, i.e., when an image is formed information about the phase is lost so that only information about intensity is available. This is often an issue in astronomy, biology, crystallography, speckle imaging, diffractive imaging where the phase of the object must be known. While there have been many approaches how to find a solution to the phase problem, numerical algorithms recovering the phase from intensity measurements become more and more popular. One of such algorithms called PhaseLift has been recently proposed. In this study, we show that even 4 masks may be sufficient for reasonable recovery of the phase. The original wavefront and th…
Merits of vision in presence of light scattering using Tiffen ProMist filters
Light scattering caused by Tiffen ProMist photographic filters of various grades at different laser radiation 640 nm, 532 nm and 405 nm was objectively studied and compared. The lack of removal of speckles from the scene of laser radiation scattering did not allow an accurate assessment of the effect of scattering on the decrease in image contrast. Subjective deterioration in visual acuity using various contrast optotypes and contrast sensitivity was assessed by scattering induction using filters up to grade #5. Vision contrast sensitivity diminishes within all studied spatial frequency range 0.5-18 cpd. The degradation of the impact factor to visual acuity without scattering filters when t…
Passive and active light scattering obstacles
Simulation of vision pathologies and adverse viewing conditions in laboratory conditions requires optical phantoms with different level of light scattering. Such obstacles are designed as passive or active elements applying several technologies. We used for studies two kinds of solid state smart materials with electrically controllable light scattering - electrooptic PLZT ceramics, polymer dispersed liquid crystals PDLC and obstacles with fixed light scattering - composite of polymer methylmethaacrilat PMM together with grinded glass microparticles. Report analyzes optical characteristics of such obstacles - attenuation, scattering, depolarization of different wavelength light at various sc…
The effect of noise, a constant background, and bit depth on the phase retrieval of pure phase objects
Acknowledgements – The authors are supported by the postdoctoral project (1.1.1.2/16/I/001; 1.1.1.2/ VIAA/1/16/199, State Education Development Agency, Republic of Latvia).
Applicability of a binary amplitude mask for creating correctors of higher-order ocular aberrations in a photoresistive layer
Ocular aberrations can be corrected with wavefront correctors created in a photoresist layer. The simplest type of the mask used in optical lithography is a binary amplitude mask. It is known that such a mask has a periodic hole pattern. The purpose of this research was to assess applicability of a binary amplitude mask for creating ocular wavefront correctors. The photoresist was applied to the substrate by using the dip-coating method. The photoresist layer was illuminated through a mask printed on a transparent film by using a laser printer. The surface of the wavefront correctors was evaluated by aberrometry, scanning electron microscopy and profilometry method. The dip-coating method c…
Model Eye Incorporating a Manually Tunable Polymer Lens and Microfluidics Chamber for Simulation of Vitreous Floaters
a model eye incorporating a manually tunable lens and microfluidics chamber for simulation of vitreous floaters has been described. Aberrations correspond to average values in population and diffraction effects could be observed.
Howard–Dolman stereovision test at different opponent colour stimuli
The purpose of this study was to investigate visual depth perception of real physical colour stimuli with red-green and blue-yellow opponency. We modified the Howard–Dolman stereotest where subjects should determine the closer of two bars emitting the light of definite colour placed in front of a CRT monitor served as colour background. Two-alternative forced-choice paradigms were used to determine depth perception threshold values. Thresholds close to 5–10 arcsec for luminance and colour contrast stimuli were revealed both for red-green and blue-yellow stimuli. A term, stereosensitivity – a reciprocal magnitude of the stereothreshold – was introduced to separately analyse luminance and col…
Measuring the refractive state of an eye based on the intensity of the retinal reflex
AbstractWhen examining the fundus of the eye with an ophthalmoscope, the intensity of the retinal reflex depends on the refractive state of the eye. In this study, application of this phenomenon for measuring the refractive state of the eye is demonstrated. First, a calibration curve relating the intensity of the retinal reflex and the refractive state was obtained using a model eye. Next, the intensity of the retinal reflex was measured while subjects were viewing a checker-board pattern, the distance of which was varied within the range from 0.17 to 1 m. Further, the relation between the refractive state and the optical power of the stimulus could be determined based on the measured light…
Color stimuli perception in presence of light scattering.
Perception of different color contrast stimuli was studied in the presence of light scattering: in a fog chamber in Clermont-Ferrand and in laboratory conditions where light scattering of similar levels was obtained, using different light scattering eye occluders. Blue (shortest wavelength) light is scattered in fog to the greatest extent, causing deterioration of vision quality especially for the monochromatic blue stimuli. However, for the color stimuli presented on a white background, visual acuity in fog for blue Landolt-C optotypes was higher than for red and green optotypes on the white background. The luminance of color Landolt-C optotypes presented on a LCD screen was chosen corresp…
FERROELECTRIC LIQUID CRYSTAL GLASSES FOR AMBLYOPIA RESEARCH
ABSTRACT Ferroelectric Liquid Crystal (FLC) filters offer the speed of electronic light shutters and the vibration free operation. These characteristics make them ideal for applications requiring short exposure times and minimal blur, offering switching times of 0.0002 of a second. FLC filters can be used as the optical shutters for machine vision purposes, also in vision research. On the basis of FLC filters we have developed ferroelectric glasses, allowing temporal separation of optical information for both eyes. In the case of amblyopia (“lazy eye”) the only way to oblige the “lazy” eye to work is to close the other better-seeing eye. We advice an appliance for dynamic visual system trai…
“SmartGlass” Obstacles for Dynamic Inducing of Light Scattering in Vision Research Experiments
We describe a technique that allows control of visual stimuli quality through the use of a setup with a polymer dispersed liquid crystal (PDLC) film positioned in the optical pathway of one or both human eyes. Nowadays, PDLC films allow alteration of the resolution and contrast limits of the transmitted light due to continuous change in the light scattering that is obtained by the application of an AC electrical field. In our experimental setup, the use of a wide-aperture up to area of 20 x 15 cm2 PDLC sheet is combined with a flat-screen PC display or with a modified display emission block without its interference filter unit and with an installed individually controllable colored light-em…
Influence of ocular aberrations on the diameter of retinal blood vessels
Lower-order and higher-order ocular aberrations influence not only visual perception but also appearance of retinal images. We suggest that the diameter of blood vessels may differ for various patterns of aberrations based on how the maps of point spread functions are orientated to these vessels. We tested our hypothesis by a star chart simulation method and by using simulating aberrations in a real image of a living retina. We observed that broadening of the blood vessels due to ocular aberrations depends not only on the relative orientation of the wavefront but also on their actual width. The change in the diameter ratio is clinically significant and is comparable to changes in the arteri…
Wavefront Recovery: Shack-Hartmann Aberrometry vs PhaseLift Using a Single Binary Amplitude Modulating Mask
We investigate whether a wavefront can be recovered by using a single mask rotated in four different positions. Results show that PhaseLift can recover the wavefront from intensity measurements using such a kind of modulation.
Optical phase retrieval using four rotated versions of a single binary amplitude modulating mask
In recent years, phase retrieval methods recovering the phase of an object from coded diffraction patterns have gained popularity. A numerical phase retrieval method called PhaseLift that recovers the phase of an object from a very limited number of coded diffraction patterns was recently proposed. Performance of PhaseLift has been analyzed for different types and the number of masks modulating an object. We present a unique application of PhaseLift that uses four rotations of a single mask, modulating only the amplitude of an object. In simulations, a phase screen with the root-mean-square (RMS) value 0.294 μm was used as the test object. The RMS value of the retrieved phase screen after …
A/V ratio as predicted by full width at half maximum and by blood vessel tracking in presence of ocular aberrations
Aberrations impact A/V ratio calculated from full width at half maximum. We investigated whether aberrations affect A/V ratio calculated by tracking the vessels. Aberrations changed the A/V ratio. We conclude that aberrations impact A/V ratio.
Portable low-cost open-source wireless spectrophotometer for fast and reliable measurements
This article is based upon COST Action CA16215 and supported by the ERDF , European-Union Project No.1.1.1.2/16/I/001.
Evaluation of retro-reflective coating performance by reflectance and perceived relative brightness measurements
Retro-reflective properties of six types and five different colors or retro-reflective materials were discussed in this paper. Reflectance optical indicatrix of samples was determined and compared to obtained psychophyisical data of perceived brightness of human observer. Microscopic structure of the retro-reflective active regions of RR's was studied. Statistically significant differences in reflectivity and brightness of various types and colors of RR's were found.
Phase retrieval for studying the structure of vitreous floaters simulated in a model eye
We simulate vitreous floaters in a model eye and apply a phase retrieval algorithm to recover the structure of the vitreous floaters. The algorithm requires modulating the object under study by a r...