0000000000223396

AUTHOR

Henrik Christensen

0000-0003-4238-4658

showing 2 related works from this author

The gut as reservoir of antibiotic resistance: microbial diversity of tetracycline resistance in mother and infant.

2011

The microbiota in the human gastrointestinal tract (GIT) is highly exposed to antibiotics, and may be an important reservoir of resistant strains and transferable resistance genes. Maternal GIT strains can be transmitted to the offspring, and resistances could be acquired from birth. This is a case study using a metagenomic approach to determine the diversity of microorganisms conferring tetracycline resistance (Tc(r)) in the guts of a healthy mother-infant pair one month after childbirth, and to investigate the potential for horizontal transfer and maternal transmission of Tc(r) genes. Fecal fosmid libraries were functionally screened for Tc(r), and further PCR-screened for specific Tc(r) …

TetracyclineMotherslcsh:MedicinePolymerase Chain ReactionMicrobiologyMicrobiology03 medical and health sciencesAntibiotic resistanceSDG 3 - Good Health and Well-beingGeneticsmedicineHumanslcsh:ScienceBiologyGene030304 developmental biologyGenetics0303 health sciencesMultidisciplinarybiology030306 microbiologyT-cell receptorlcsh:RInfant NewbornTetracycline ResistanceInfantComputational BiologyBacteroidetesbiology.organism_classification3. Good healthGastrointestinal TractFosmidComposite transposonHorizontal gene transfer/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_beinglcsh:QResearch Articlemedicine.drugPLoS ONE
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Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes

2018

Advancement of DNA sequencing technology allows the routine use of genome sequences in the various fields of microbiology. The information held in genome sequences proved to provide objective and reliable means in the taxonomy of prokaryotes. Here, we describe the minimal standards for the quality of genome sequences and how they can be applied for taxonomic purposes.

0301 basic medicineWhole genome sequencing030106 microbiologyPhylogenomicsGeneral MedicineComputational biologyGenomicsSequence Analysis DNAMinimal standardsAverage nucleotide identityBiologyMicrobiologyGenomeDNA sequencing03 medical and health sciencesProkaryotic CellsPhylogenomicsTerminology as TopicGenome sequenceTaxonomy (biology)Prokaryotic taxonomyEcology Evolution Behavior and SystematicsPhylogeny
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