Terrestrial organic matter quantity or decomposition state does not compensate for its poor nutritional quality for Daphnia

Inferring Phytoplankton, Terrestrial Plant and Bacteria Bulk δ¹³C Values from Compound Specific Analyses of Lipids and Fatty Acids.

Stable isotope mixing models in aquatic ecology require δ13C values for food web end members such as phytoplankton and bacteria, however it is rarely possible to measure these directly. Hence there is a critical need for improved methods for estimating the δ13C ratios of phytoplankton, bacteria and terrestrial detritus from within mixed seston. We determined the δ13C values of lipids, phospholipids and biomarker fatty acids and used these to calculate isotopic differences compared to the whole-cell δ13C values for eight phytoplankton classes, five bacterial taxa, and three types of terrestrial organic matter (two trees and one grass). The lipid content was higher amongst the phytoplankton (…

Retroconversion of docosapentaenoic acid (n-6): an alternative pathway for biosynthesis of arachidonic acid in Daphnia magna.

The aim of this study was to assess metabolic pathways for arachidonic acid (20:4n-6) biosynthesis in Daphnia magna. Neonates of D. magna were maintained on [13C] enriched Scenedesmus obliquus and supplemented with liposomes that contained separate treatments of unlabeled docosapentaenoic acid (22:5n-6), 20:4n-6, linoleic acid (18:2n-6) or oleic acid (18:1n-9). Daphnia in the control treatment, without any supplementary fatty acids (FA) containing only trace amounts of 20:4n-6 (~0.3 % of all FA). As expected, the highest proportion of 20:4n-6 (~6.3 %) was detected in Daphnia that received liposomes supplemented with this FA. Higher availability of 18:2n-6 in the diet increased the proportio…

A Fatty Acid Based Bayesian Approach for Inferring Diet in Aquatic Consumers

We modified the stable isotope mixing model MixSIR to infer primary producer contributions to consumer diets based on their fatty acid composition. To parameterize the algorithm, we generated a 'consumer-resource library' of FA signatures of Daphnia fed different algal diets, using 34 feeding trials representing diverse phytoplankton lineages. This library corresponds to the resource or producer file in classic Bayesian mixing models such as MixSIR or SIAR. Because this library is based on the FA profiles of zooplankton consuming known diets, and not the FA profiles of algae directly, trophic modification of consumer lipids is directly accounted for. To test the model, we simulated hypothet…

Diet-specific biomarkers show that high-quality phytoplankton fuels herbivorous zooplankton in large boreal lakes

SUMMARY 1. The zooplankton is a key link in the transfer of energy from primary producers up through aquatic food webs. Previous efforts to quantify the importance of basal resources to aquatic consumers have used stable isotopes (SI) and simple ternary models, including only ‘bulk’ phytoplankton, bacteria or terrestrial particulate organic matter (t-POM). 2. We used a novel Bayesian mixing model based on fatty acids (FA) to quantify the dietary assimilation of seven basal resources, including five phytoplankton groups, pelagic bacteria and t-POM, to Cladocera in large boreal lakes in Finland. To account for trophic enrichment of FA from the diet to consumers, we parameterised the model wit…

The influence of bacteria-dominated diets on Daphnia magna somatic growth, reproduction, and lipid composition

We explored how dietary bacteria affect the life history traits and biochemical composition of Daphnia magna, using three bacteria taxa with very different lipid composition. Our objectives were to (1) examine whether and how bacteria-dominated diets affect Daphnia survival, growth, and fecundity, (2) see whether bacteria-specific fatty acid (FA) biomarkers accrued in Daphnia lipids, and (3) explore the quantitative relationship between bacteria availability in Daphnia diets and the amounts of bacterial FA in their lipids. Daphnia were fed monospecific and mixed diets of heterotrophic (Micrococcus luteus) or methanotrophic bacteria (Methylomonas methanica and Methylosinus trichosporium) and…

DifferingDaphnia magnaassimilation efficiencies for terrestrial, bacterial, and algal carbon and fatty acids

There is considerable interest in the pathways by which carbon and growth-limiting elemental and biochemical nutrients are supplied to upper trophic levels. Fatty acids and sterols are among the most important molecules transferred across the plant-animal interface of food webs. In lake ecosystems, in addition to phytoplankton, bacteria and terrestrial organic matter are potential trophic resources for zooplankton, especially in those receiving high terrestrial organic matter inputs. We therefore tested carbon, nitrogen, and fatty acid assimilation by the crustacean Daphnia magna when consuming these resources. We fed Daphnia with monospecific diets of high-quality (Cryptomonas marssonii) a…

How important are terrestrial organic carbon inputs for secondary production in freshwater ecosystems?

Article

Combined effects of eutrophication and warming on polyunsaturated fatty acids in complex phytoplankton communities:A mesocosm experiment

Climate change and eutrophication are among the main stressors of shallow freshwater ecosystems, and their effects on phytoplankton community structure and primary production have been studied extensively. However, their combined effects on the algal production of polyunsaturated fatty acids (PUFA), specifically, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are currently unresolved. Moreover, the proximate reasons for changes in phytoplankton EPA and DHA concentrations are unclear, i.e., the relative importance of ecological (changes in the community composition) vs. ecophysiological (within taxa changes in EPA and DHA levels) factors. We investigated the responses of phytopla…

Differing Daphnia magna assimilation efficiencies for terrestrial, bacterial, and algal carbon and fatty acids

There is considerable interest in the pathways by which carbon and growthlimiting elemental and biochemical nutrients are supplied to upper trophic levels. Fatty acids and sterols are among the most important molecules transferred across the plant–animal interface of food webs. In lake ecosystems, in addition to phytoplankton, bacteria and terrestrial organic matter are potential trophic resources for zooplankton, especially in those receiving high terrestrial organic matter inputs. We therefore tested carbon, nitrogen, and fatty acid assimilation by the crustacean Daphnia magna when consuming these resources. We fed Daphnia with monospecific diets of high-quality (Cryptomonas marssonii) an…

Inferring phytoplankton community composition with a fatty acid mixing model

The taxon specificity of fatty acid composition in algal classes suggests that fatty acids could be used as chemotaxonomic markers for phytoplankton composition. The applicability of phospholipid-derived fatty acids as chemotaxonomic markers for phytoplankton composition was evaluated by using a Bayesian fatty acid-based mixing model. Fatty acid profiles from monocultures of chlorophytes, cyanobacteria, diatoms, euglenoids, dinoflagellates, raphidophyte, cryptophytes and chrysophytes were used as a reference library to infer phytoplankton community composition in five moderately humic, large boreal lakes in three different seasons (spring, summer and fall). The phytoplankton community compo…

Fatty acid composition as biomarkers of freshwater microalgae: analysis of 37 strains of microalgae in 22 genera and in seven classes

The fatty acid (FA) composition of algae is an important determinant of their food quality for consumers, and FAs can also be used as biomarkers for biochemical and energetic pathways in food webs. FA analyses of 7 freshwater algal classes and 37 strains showed clear similarity within classes and strong differences amongst classes. Class was a dominant factor (66.4%) explaining variation in FA signatures of microalgae. The 7 algal classes comprised 4 separate groups according to their FA profiles: (1) Chlorophyceae and Trebouxiophyceae, (2) Bacillariophyceae, (3) Cryptophyceae, Chrysophyceae, and Raphidophyceae, and (4) Euglenophyceae. Each group had a characteristic FA composition, althoug…

Lake zooplankton delta13C values are strongly correlated with the delta13C values of distinct phytoplankton taxa

Article

Lake zooplankton δ13C values are strongly correlated with the δ13C values of distinct phytoplankton taxa

Analyses of carbon stable isotopes are often used to estimate the contributions of allochthonous and autochthonous dietary resources to aquatic consumers. Most pelagic food web studies assume that all phytoplankton taxa have a similar δ13C value. We studied pelagic food web compartments (dissolved inorganic carbon [DIC], phytoplankton, bacteria, seston, cladoceran zooplankton) in 12 small (< 0.1 km2) lakes in southern Finland. These lakes were classified as oligotrophic, mesotrophic, eutrophic, and dystrophic based on their concentrations of total phosphorus and dissolved organic carbon. Additionally, we studied phytoplankton photosynthetic carbon fractionation (εp) in laboratory conditions…

Appendix C. Results of Cox regression survival analysis of experiments.

Results of Cox regression survival analysis of experiments.

Appendix E. Principal component analysis of diets and Daphnia.

Principal component analysis of diets and Daphnia.

Appendix A. Detailed concentration information of added nutrients in batch and life table experiments.

Detailed concentration information of added nutrients in batch and life table experiments.

Appendix B. Fatty acid and sterol profiles of terrestrial particulate organic carbon (t-POC), bacteria, and phytoplankton diets.

Fatty acid and sterol profiles of terrestrial particulate organic carbon (t-POC), bacteria, and phytoplankton diets.

Appendix D. Correlations between different nutrients of diets and offspring and body size of Daphnia.

Correlations between different nutrients of diets and offspring and body size of Daphnia.

Appendix F. The parameter estimates of t-POC diets for power functions.

The parameter estimates of t-POC diets for power functions.