0000000000231047
AUTHOR
Géraldine Offer
PO39 La fission mitochondriale induite par le glucose est indispensable à la signalisation ROS lors de la détection hypothalamique de l’hyperglycémie
Introduction Nous avons montre que la detection hypothalamique de l’hyperglycemie (HG) fait intervenir une signalisation ROS d’origine mitochondriale, laquelle est indispensable aux reponses effectrices, dont la secretion d’insuline. Par ailleurs, des etudes recentes montrent que la morphologie des mitochondries varie lors de l’HG, morphologie qui est dependante de la dynamique mitochondriale, modifiant alors la production de ROS. Ainsi, l’inhibition de la fission mitochondriale diminue la production de ROS lors d’une hyperglycemie dans l’hepatocyte par exemple. Objectif Comprendre si, in vivo, la signalisation par les ROS lors du « glucose sensing » hypothalamique, implique la dynamique mi…
Enhanced Hypothalamic Glucose Sensing in Obesity: Alteration of Redox Signalling
1939-327X (Electronic) Journal article; Objective : Recent data demonstrate that glucose sensing in different tissues is initiated by an intracellular redox-signaling pathway in physiological conditions. However, the relevance of such a mechanism in metabolic disease is not known. The aim of the present study was to determine whether brain-glucose hypersensitivity present in obese Zucker rat is related to an alteration in redox signaling. Research design and Methods: Brain glucose sensing alteration was investigated in vivo through the evaluation of electrical activity in arcuate nucleus, changes in ROS levels, and hypothalamic glucose-induced insulin secretion. In basal conditions, modific…
Importance of mitochondrial dynamin-related protein 1 in hypothalamic glucose sensitivity in rats.
International audience; AIMS: Hypothalamic mitochondrial reactive oxygen species (mROS)-mediated signaling has been recently shown to be involved in the regulation of energy homeostasis. However, the upstream signals that control this mechanism have not yet been determined. Here, we hypothesize that glucose-induced mitochondrial fission plays a significant role in mROS-dependent hypothalamic glucose sensing. RESULTS: Glucose-triggered translocation of the fission protein dynamin-related protein 1 (DRP1) to mitochondria was first investigated in vivo in hypothalamus. Thus, we show that intracarotid glucose injection induces the recruitment of DRP1 to VMH mitochondria in vivo. Then, expressio…