0000000000239902
AUTHOR
J. D. Beck
Determination of DNase activity in the sweat and the urine
Die Mikrodiskelektrophorese mit Polyacrylamidgelen wird als neue Methode zum Nachweis von Desoxyribonucleasen im Schweis beschrieben. Unterschiede der Nuclease-Aktivitat im Schweis und Urin gesunder und an Mucoviscidose erkrankter Kinder ergaben sich nicht. Die hochste Aktivitat im Schweis findet sich nach saurer Inkubation (pH 5,0) unter Zusatz von EDTA. Nach Inkubation in schwach alkalischem Milieu (pH 7,4) und Ionenzusatz kann nur hochstens 1 enzymaktive Bande nachgewiesen werden. Im Urin lassen sich durch saure Inkubation (pH 5,0) und Magnesium- und Calciumionenzusatz sowohl bei Patienten als auch bei Kontrollpersonen 4 Nuclease-Banden deutlich darstellen. Die Ergebnisse werden im Zusam…
Reversible graft versus host reaction as cause of erythrophagic splenomegaly in a child?
The case history of a 9 months old infant with hepatosplenomegaly, pancytopnaenia and disturbances of clotting and cellular immune reactivity is reported. The spleen was removed and showed striking erythrophagocytosis by proliferating histiocytes, typical of "familial erythrophagocytic reticulosis" (Farquhar). A graft-versus-host reaction is discussed as a possible underlying cause. The favourable clinical course and full recovery point to an interrelation with primary hypersplenism.
Communication and ethical considerations for fertility preservation for patients with childhood, adolescent, and young adult cancer
Patients with childhood, adolescent, and young adult cancer who will be treated with gonadotoxic therapies are at increased risk for infertility. Many patients and their families desire biological children but effective communication about treatment-related infertility risk and procedures for fertility preservation does not always happen. The PanCareLIFE Consortium and the International Late Effects of Childhood Cancer Guideline Harmonization Group reviewed the literature and developed a clinical practice guideline that provides recommendations for ongoing communication methods for fertility preservation for patients who were diagnosed with childhood, adolescent, and young adult cancer at a…