0000000000243686
AUTHOR
Felix Sommer
The gut microbiota instructs the hepatic endothelial cell transcriptome
Summary The gut microbiota affects remote organ functions but its impact on organotypic endothelial cell (EC) transcriptomes remains unexplored. The liver endothelium encounters microbiota-derived signals and metabolites via the portal circulation. To pinpoint how gut commensals affect the hepatic sinusoidal endothelium, a magnetic cell sorting protocol, combined with fluorescence-activated cell sorting, was used to isolate hepatic sinusoidal ECs from germ-free (GF) and conventionally raised (CONV-R) mice for transcriptome analysis by RNA sequencing. This resulted in a comprehensive map of microbiota-regulated hepatic EC-specific transcriptome profiles. Gene Ontology analysis revealed that …
High-resolution laser spectroscopy of Al27–32
Hyperfine spectra of $^\text{27-32}$Al ($Z=13$) have been measured at the ISOLDE-CERN facility via collinear laser spectroscopy using the $3s^23p\ ^2\text{P}^\text{o} _{3/2}\rightarrow 3s^24s\ ^2\text{S}_{1/2}$ atomic transition. For the first time, mean-square charge radii of radioactive aluminum isotopes have been determined alongside the previously unknown magnetic dipole moment of $^{29}$Al and electric quadrupole moments of $^{29,30}$Al. A potentially reduced charge radius at $N=19$ may suggest an effect of the $N=20$ shell closure, which is visible in the Al chain, contrary to other isotopic chains in the $sd$ shell. The experimental results are compared to theoretical calculations in…
The Microbiota Promotes Arterial Thrombosis in Low-Density Lipoprotein Receptor-Deficient Mice
Our results demonstrate a functional role for the commensal microbiota in atherothrombosis. In a ferric chloride injury model of the carotid artery, GF C57BL/6J mice had increased occlusion times compared to colonized controls. Interestingly, in late atherosclerosis, HFD-fed GF Ldlr−/− mice had reduced plaque rupture-induced thrombus growth in the carotid artery and diminished ex vivo thrombus formation under arterial flow conditions.
High-resolution laser spectroscopy of Al27-32
Hyperfine spectra of Al27-32 (Z=13) have been measured at the ISOLDE-CERN facility via collinear laser spectroscopy using the 3s23p2P3/2o→3s24s2S1/2 atomic transition. For the first time, mean-square charge radii of radioactive aluminum isotopes have been determined alongside the previously unknown magnetic dipole moment of Al29 and electric quadrupole moments of Al29,30. A potentially reduced charge radius at N=19 may suggest an effect of the N=20 shell closure, which is visible in the Al chain, contrary to other isotopic chains in the sd shell. The experimental results are compared with theoretical calculations in the framework of the valence-space in-medium similarity renormalization gro…