0000000000247535

AUTHOR

Bettina Krieg

showing 3 related works from this author

Cationic Nanohydrogel Particles as Potential siRNA Carriers for Cellular Delivery

2012

Oligonucleotides such as short, double-stranded RNA (siRNA) or plasmid DNA (pDNA) promise high potential in gene therapy. For pharmaceutical application, however, adequate drug carriers are required. Among various concepts progressing in the market or final development, nanosized hydrogel particles may serve as novel transport media especially for siRNA. In this work, a new concept of synthesizing polymeric cationic nanohydrogels was developed, which offers a promising strategy to complex and transport siRNA into cells. For this purpose, amphiphilic reactive ester block copolymers were synthesized by RAFT polymerization of pentafluorophenyl methacrylate as reactive ester monomer together wi…

Models MolecularMaterials scienceMolecular ConformationGeneral Physics and AstronomyMethacrylateCell Linechemistry.chemical_compoundAmphiphilePolymer chemistryAnimalsGeneral Materials ScienceReversible addition−fragmentation chain-transfer polymerizationAminesRNA Small Interferingchemistry.chemical_classificationDrug CarriersGeneral EngineeringCationic polymerizationBiological TransportEstersHydrogelsPolymerCombinatorial chemistryNanostructuresRatsMonomerchemistrySolventsDrug carrierHydrophobic and Hydrophilic InteractionsEthylene glycolACS Nano
researchProduct

The guanidinium group as a key part of water-soluble polymer carriers for siRNA complexation and protection against degradation.

2014

Here, the preparation of a novel block copolymer consisting of a statistical copolymer N-(2-hydroxypropyl) methacrylamide-s-N-(3-aminopropyl) methacrylamide and a short terminal 3-guanidinopropyl methacrylamide block is reported. This polymer structure forms neutral but water-soluble nanosized complexes with siRNA. The siRNA block copolymer complexes are first analyzed using agarose gel electrophoresis and their size is determined with fluorescence correlation spectroscopy. The protective properties of the polymer against RNA degradation are investigated by treating the siRNA block copolymer complexes with RNase V1. Heparin competition assays confirm the efficient release of the cargo in vi…

chemistry.chemical_classificationAcrylamidesMaterials sciencePolymers and PlasticsMicroscale thermophoresisRNase PPolymersOrganic ChemistryWaterFluorescence correlation spectroscopyPolymerchemistry.chemical_compoundchemistryAgarose gel electrophoresisPolymer chemistryEndoribonucleasesMaterials ChemistryCopolymerMethacrylamideMoleculeRNA Small InterferingGuanidineMacromolecular rapid communications
researchProduct

A modified guanosine phosphoramidite for click functionalization of RNA on the sugar edge

2012

A propargyl containing guanosine phosphoramidite was synthesized and incorporated into siRNA, enabling click-ligation with an azido fluorophore onto the nucleobase sugar edge. Duplex stability was not affected by labeling at this new site, which allowed deconvolution of the effects of label, structure and attachment site on RNAi activity.

PhosphoramiditeFluorophoreGuanosineMolecular StructureCarbohydratesMetals and AlloysGuanosineRNAGeneral ChemistryCombinatorial chemistryCatalysisSurfaces Coatings and FilmsElectronic Optical and Magnetic MaterialsNucleobasechemistry.chemical_compoundOrganophosphorus CompoundschemistryDuplex (building)PropargylMaterials ChemistryCeramics and CompositesRNASurface modificationClick ChemistryRNA Small InterferingChemical Communications
researchProduct