6533b82bfe1ef96bd128d7f7
RESEARCH PRODUCT
A modified guanosine phosphoramidite for click functionalization of RNA on the sugar edge
Mark HelmSalifu Seidu-larryMarkus HirschBettina KriegOlwen Domingosubject
PhosphoramiditeFluorophoreGuanosineMolecular StructureCarbohydratesMetals and AlloysGuanosineRNAGeneral ChemistryCombinatorial chemistryCatalysisSurfaces Coatings and FilmsElectronic Optical and Magnetic MaterialsNucleobasechemistry.chemical_compoundOrganophosphorus CompoundschemistryDuplex (building)PropargylMaterials ChemistryCeramics and CompositesRNASurface modificationClick ChemistryRNA Small Interferingdescription
A propargyl containing guanosine phosphoramidite was synthesized and incorporated into siRNA, enabling click-ligation with an azido fluorophore onto the nucleobase sugar edge. Duplex stability was not affected by labeling at this new site, which allowed deconvolution of the effects of label, structure and attachment site on RNAi activity.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2012-10-06 | Chemical Communications |