Additional file 1: Table S1. of Apolipoprotein B is regulated by gonadotropins and constitutes a predictive biomarker of IVF outcomes

Patients' characteristics. All values are reported by the mean ± SD (Standard Deviation) or the number of subjects (percentage of the total). BMI, Body Mass Index; IVF, In Vitro Fertilization; ICSI, Intracytoplasmic Sperm Injection. (DOCX 12 kb)

Additional file 2: Table S2. of Apolipoprotein B is regulated by gonadotropins and constitutes a predictive biomarker of IVF outcomes

Description of patients' characteristics according to APOB concentrations divided into quartiles. Q1, quartile 1; Q2, quartile 2; Q3, quartile 3; Q4, quartile 4; SD, Standard Deviation; CI, Confidence Intervals, BMI, Body Mass Index; IVF, In Vitro Fertilization; ICSI, Intracytoplasmic Sperm Injection. (DOCX 15Â kb)

Similarly increased congenital anomaly rates after intrauterine insemination and IVF technologies: a retrospective cohort study

International audience; BACKGROUND: While intrauterine insemination (IUI), a simple, inexpensive and non-invasive technique, is the most used assisted reproduction technology (ART) worldwide, the risk of major birth defects following IUI is paradoxically not well documented. METHODS: Retrospective cohort study performed in Burgundy, France, over a 9-year period which consisted of the cross analysis of two prospective databases, the Burgundy perinatal network database and the database of the assisted conception units in Burgundy. A total of 1348 ART singletons [in vitro fertilization technologies (IVFT): n= 903; IUI: n= 445] matched with 4044 infants conceived naturally, 552 ART twins (IVFT:…

Male infertility: an obstacle to sexuality?

International audience; Interactions between infertility and sexuality are numerous and complex. Infertile men may suffer from sexual dysfunction (SD) when undergoing an assisted reproductive technology programme. We undertook a review both in French and English of the available data on male SD when being diagnosed with a fertility problem with a specific focus on azoospermic men. The review was performed over a 30-year time period using PubMed/Medline. The sexual concerns and needs of infertile/sterile men for whom potential parenting can be compromised were evaluated. When diagnosed with infertility, men usually go through a crisis that can have a deleterious effect on their sexuality wit…

Outcomes with intracytoplasmic sperm injection of cryopreserved sperm from men with spinal cord injury

Erectile dysfunction, ejaculatory dysfunction and poor semen quality are the main causes of infertility in men with spinal cord injury (SCI). Different sperm retrieval techniques such as penile vibratory stimulation (PVS), electro-ejaculation (EEJ) or surgical sperm retrieval (SSR) associated or not with sperm cryopreservation can be offered to these patients to preserve their fertility. If fatherhood cannot be achieved naturally, assisted reproductive techniques can be offered to these patients using either fresh or frozen/thawed sperm. The aim of the study was to report in SCI patients from Dijon (Burgundy) and in the literature, intracytoplasmic sperm injection (ICSI) outcomes using froz…

Apolipoprotein B is regulated by gonadotropins and constitutes a predictive biomarker of IVF outcomes

Background Follicular fluid (FF) is an important micro-environment influencing oocyte growth, its development competence, and embryo viability. The FF content analysis allows to identify new relevant biomarkers, which could be predictive of in vitro fertilization (IVF) outcomes. Inside ovarian follicle, the amount of FF components from granulosa cells (GC) secretion, could be regulated by gonadotropins, which play a major role in follicle development. Methods This prospective study included 61 female undergoing IVF or Intra-cytoplasmic sperm injection (ICSI) procedure. Apolipoprotein B (APOB) concentrations in follicular fluid and APOB gene and protein expression in granulosa cells from rep…

Characterization of cryoprotectants ternary mixture according to cooling rates

How to demonstrate that eSET does not compromise the likelihood of having a baby?

BACKGROUND: In several randomized studies, elective single embryo transfer (eSET) has proven its effectiveness in reducing twin pregnancy rates while obtaining acceptable overall pregnancy rates. However, there is no outcome measurement consensus to evaluate the effectiveness of eSET versus double-embryo transfer (DET). METHODS: This study evaluated whether or not adopting an eSET strategy instead of a DET strategy lowers the probability of having at least one live-born infant in good prognosis couples. Seven hundred and twenty-six couples were divided into two groups. The retrospective arm of the study was undertaken on the first group of couples (n = 483, DET group) and the prospective ar…

Impact on ICSI outcomes of adding 24 h of in vitro culture before testicular sperm freezing: a retrospective study.

To compare sperm parameters and intracytoplasmic sperm injection (ICSI) outcomes for testicular spermatozoa frozen on the day of the biopsy (DO) with those frozen after 24 h of in vitro culture (D1).In this retrospective study, from 1999 to 2012, forty-nine azoospermic patients were included to compare sperm (motility and viability) and outcomes (fertilization (FR), implantation (IR), pregnancy (PR) and delivery rates (DR)).The in vitro culture increased total motility (+2.8 %, p = 0.0161) but decreased viability (-8.3 %, p = 0.007). After 24 h of culture, the post-thaw changes in motility and viability were not significant. Twenty-six couples underwent ICSI: thirty-four ICSI were performed…

Additional file 3: Figure S1. of Apolipoprotein B is regulated by gonadotropins and constitutes a predictive biomarker of IVF outcomes

17 β-estradiol quantification (ng/l) into culture medium by immuno-chemiluminescence. Androstenedione (And) was added to the culture medium to provide specific substrate to granulosa cells, for oestrogen synthesis. (TIF 69 kb)

Additional file 3: Figure S1. of Apolipoprotein B is regulated by gonadotropins and constitutes a predictive biomarker of IVF outcomes

17 β-estradiol quantification (ng/l) into culture medium by immuno-chemiluminescence. Androstenedione (And) was added to the culture medium to provide specific substrate to granulosa cells, for oestrogen synthesis. (TIF 69 kb)

Additional file 4: Figure S2. of Apolipoprotein B is regulated by gonadotropins and constitutes a predictive biomarker of IVF outcomes

The constant GAPDH gene expression in GC pools (n = 10) under our experiment conditions. All Ct-values were reported for the different GC pools from FF samples. (JPG 137 kb)

Additional file 4: Figure S2. of Apolipoprotein B is regulated by gonadotropins and constitutes a predictive biomarker of IVF outcomes

The constant GAPDH gene expression in GC pools (n = 10) under our experiment conditions. All Ct-values were reported for the different GC pools from FF samples. (JPG 137 kb)