0000000000275486
AUTHOR
W. Puppe
Comparison of the BD Directigen Flu A+B Kit and the Abbott TestPack RSV with a multiplex RT-PCR ELISA for rapid detection of influenza viruses and respiratory syncytial virus
ABSTRACTThe Directigen Flu A+B enzyme immunoassay and the Abbott TestPack RSV enzyme immunoassay were each compared with a multiplex RT-PCR ELISA by testing 635 nasopharyngeal aspirates collected from children aged < 16 years who had been hospitalised with acute respiratory tract infection during the epidemic season 2002–2003. In this study, the sensitivity of the Directigen Flu A+B assay was unacceptably low (29.3% and 10.0%, respectively) for the detection of influenza A and B viruses. The sensitivity of the Abbott TestPack RSV assay (77.4%) was acceptable and in agreement with the multiplex RT-PCR ELISA.
Respiratorisches-Syncytial-Virus
Die Arbeitsgemeinschaft Influenza (AGI) erfasst seit 1992 in den Winterhalbjahren das Auftreten von akuten respiratorischen Erkrankungen (ARE) mit einem Netzwerk aus primarversorgenden Praxen. Zusatzlich werden in einem Teil der Praxen stichprobenartig Abstriche entnommen und in dem Nationalen Referenzzentrum fur Influenza und anderen Labors auf Influenzaviren untersucht. Im Winter 1998/99 wurde in einem Netzwerk aus 550 Arztpraxen bundesweit eine Erhohung der Morbiditat akuter Atemwegserkrankungen beobachtet, die nicht durch Influenza verursacht wurde. Um die Ursache zu ergrunden, wurden weitere verfugbare Daten wie die Beobachtungen des Laborberichtssystems fur respiratorische Erkrankunge…
The incidence of influenza-associated hospitalizations in children in Germany
Since new vaccines and anti-viral drugs for influenza have become available, collation of actual and country-specific epidemiological data is essential. Since respiratory syncytial virus (RSV) is a well known paediatric airway pathogen and some epidemiological data exist already, a comparison between influenza and RSV seems warranted. From July 1996 to June 2001 the naso-pharyngeal aspirates (NPA) of children from birth to 16 years of age, admitted to one of the two paediatric hospitals in Kiel, Germany, were investigated by a nine-valent multiplex reverse transcriptase PCR assay. NPA were investigated in 60·8% of 3469 children admitted with an acute respiratory tract infection. Community-a…
Evaluation of a multiplex reverse transcriptase PCR ELISA for the detection of nine respiratory tract pathogens.
A multiplex reverse transcription (RT) polymerase chain reaction combined with a microwell hybridization assay (m-RT-PCR-ELISA) was previously developed to detect nine different microorganisms: enterovirus (EV), influenza virus type A (IVA) and type B (IVB), respiratory syncytial virus (RSV), parainfluenzavirus type 1 (PIV1) and type 3 (PIV3), adenovirus (AV), Mycoplasma pneumoniae (Mpn), Chlamydia pneumoniae (Cpn) in a single test. These organisms do not usually colonize the respiratory tract of humans, but, if present, it may be assumed they are involved in respiratory disease.The m-RT-PCR-ELISA was tested on (i) culture supernatants of unknown contents, (ii) by determining the analytical…