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RESEARCH PRODUCT

Comparison of the BD Directigen Flu A+B Kit and the Abbott TestPack RSV with a multiplex RT-PCR ELISA for rapid detection of influenza viruses and respiratory syncytial virus

Heinz-josef SchmittBritta GröndahlW. PuppeJ. A. I. WeiglJ. A. I. Weigl

subject

Microbiology (medical)Paramyxoviridaerespiratory syncytial virusrespiratory tract infectionEnzyme-Linked Immunosorbent AssayPolymerase Chain ReactionSensitivity and Specificityinfluenza virusVirusAssaysMicrobiologyPneumovirinaeNasopharynxMultiplex polymerase chain reactionmedicineMultiplexProspective StudiesMononegaviralesbiologymedicine.diagnostic_testReverse Transcriptase Polymerase Chain Reactionvirus diseasesGeneral Medicinebiology.organism_classificationenzyme immunoassayVirologyInfluenza B virusInfectious Diseasesmultiplex RT-PCRInfluenza A virusRespiratory Syncytial Virus HumanImmunoassayReagent Kits DiagnosticViral disease

description

ABSTRACTThe Directigen Flu A+B enzyme immunoassay and the Abbott TestPack RSV enzyme immunoassay were each compared with a multiplex RT-PCR ELISA by testing 635 nasopharyngeal aspirates collected from children aged < 16 years who had been hospitalised with acute respiratory tract infection during the epidemic season 2002–2003. In this study, the sensitivity of the Directigen Flu A+B assay was unacceptably low (29.3% and 10.0%, respectively) for the detection of influenza A and B viruses. The sensitivity of the Abbott TestPack RSV assay (77.4%) was acceptable and in agreement with the multiplex RT-PCR ELISA.

yearjournalcountryeditionlanguage
2005-10-01Clinical Microbiology and Infection
https://doi.org/10.1111/j.1469-0691.2005.01223.x