0000000000275802

AUTHOR

Camelia Diguta

showing 3 related works from this author

Non-Botrytis grape-rotting fungi responsible for earthy and moldy off-flavors and mycotoxins

2012

Abstract The grape microflora is complex and includes filamentous fungi, yeasts and bacteria with different physiological characteristics and effects on wine production. Most studies have focused on the wine microbiota, but a few studies have reported the ecology of grape microorganisms. Some of these organisms — such as non-Botrytis bunch rotting fungi, which greatly influence the safety or sensory quality of wine, due to the production of mycotoxins and off-flavors, respectively — are considered to be spoilage agents. We review here the diversity of filamentous fungi on grapes and the factors influencing their development, such as grape ripening stage, environmental factors (climate, rain…

food.ingredientMicroorganismPopulationFood spoilageWineMicrobiologychemistry.chemical_compoundfoodBotanyHumansVitisFood scienceeducationMycotoxinBotrytisWineeducation.field_of_studyAspergillusbiologyfungiFungifood and beveragesMycotoxinsbiology.organism_classificationchemistryTasteFermentationPenicilliumFood ScienceFood Microbiology
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PCR ITS-RFLP: A useful method for identifying filamentous fungi isolates on grapes.

2010

Restriction digestion analysis of the ITS products was tested as an easy method to identify isolates of filamentous fungi on grapes. Endonucleases SduI, HinfI, MseI, HaeIII were used. Endonucleases BfmI, Cfr9I, Hpy188I, MaeII or PspGI were used as necessary to complete discrimination. The 43 species studied generated 42 different composite profiles. Only the species P. thomii and P. glabrum gave the same composite profile. 96.3% strains tested could be differentiated to the species level with only four enzymes. Hundred ninety nine strains of filamentous fungi were isolated from various vineyards in Burgundy and identified by this method. Penicillium (58.5%) was the genus the most frequently…

MicrobiologyVineyardPolymerase Chain ReactionMicrobiologylaw.inventionHaeIIIFungal ProteinsGenuslawBotanymedicineVitisAmplified Fragment Length Polymorphism AnalysisMycological Typing TechniquesPolymerase chain reactionAspergillusbiologyFungibiology.organism_classificationPenicilliumRestriction digestDNA IntergenicRestriction fragment length polymorphismPolymorphism Restriction Fragment LengthFood Sciencemedicine.drugFood microbiology
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Development of  a qPCR assay for specific quantification of Botrytis cinerea on grapes

2010

The aim of this study was to develop a system for rapid and accurate real-time quantitative PCR (qPCR) identification and quantification of Botrytis cinerea , one of the major pathogens present on grapes. The intergenic spacer (IGS) region of the nuclear ribosomal DNA was used to specifically detect and quantify B. cinerea . A standard curve was established to quantify this fungus. The qPCR reaction was based on the simultaneous detection of a specific IGS sequence and also contained an internal amplification control to compensate for variations in DNA extraction and the various compounds from grapes that inhibit PCR. In these conditions, the assay had high efficiency (97%), and the limit o…

food.ingredientbiologyfungibiology.organism_classificationMicrobiologyDNA extractionMolecular biologylaw.inventionReverse transcription polymerase chain reactionfoodReal-time polymerase chain reactionIntergenic regionlawGeneticsMolecular BiologyRibosomal DNAPolymerase chain reactionBotrytisBotrytis cinereaFEMS Microbiology Letters
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