Combined multi-wavelength laser speckle contrast imaging and diffuse reflectance imaging for skin perfusion assessment

Abstract Laser Speckle Contrast Imaging (LSCI) is a powerful low-cost method for visualization of flow, microcirculation and blood perfusion. Due to the fact that diseased and healthy tissues has different blood perfusion, LSCI can be a perspective tool for cancer diagnostics and discrimination between different types of tissues. Previously, multispectral diffuse reflectance imaging method for melanoma diagnostics has been introduced. In this work, multi-wavelength (532-, 655- and 850- nm) LSCI technique combined with hyperspectral camera and diffuse reflectance imaging method will be used for assessment of tissues with different skin perfusion properties. An in vivo experiment with occlusi…