0000000000286418
AUTHOR
Igor Meglinski
showing 5 related works from this author
Combined multi-wavelength laser speckle contrast imaging and diffuse reflectance imaging for skin perfusion assessment
2019
Abstract Laser Speckle Contrast Imaging (LSCI) is a powerful low-cost method for visualization of flow, microcirculation and blood perfusion. Due to the fact that diseased and healthy tissues has different blood perfusion, LSCI can be a perspective tool for cancer diagnostics and discrimination between different types of tissues. Previously, multispectral diffuse reflectance imaging method for melanoma diagnostics has been introduced. In this work, multi-wavelength (532-, 655- and 850- nm) LSCI technique combined with hyperspectral camera and diffuse reflectance imaging method will be used for assessment of tissues with different skin perfusion properties. An in vivo experiment with occlusi…
Assessment of age-related skin changes using hyperspectral polarization imaging
2019
Abstract We report a development of hyperspectral polarization imaging approach for assessment of age-related skin changes. The degree of residual polarization is used as a quantitative marker of the age-related pathological malformations. The developed approach in frame of express analysis of the degree of residual polarization in each pixel of the image are shown.
Optogenetic Control of Bacterial Expression by Red Light
2022
In optogenetics, as in nature, sensory photoreceptors serve to control cellular processes by light. Bacteriophytochrome (BphP) photoreceptors sense red and far-red light via a biliverdin chromophore and, in response, cycle between the spectroscopically, structurally, and functionally distinct Pr and Pfr states. BphPs commonly belong to two-component systems that control the phosphorylation of cognate response regulators and downstream gene expression through histidine kinase modules. We recently demonstrated that the paradigm BphP from Deinococcus radiodurans exclusively acts as a phosphatase but that its photosensory module can control the histidine kinase activity of homologous receptors.…
The use of Stokes-Mueller polarimetry for assessment of amyloid-β progression in a mouse model of Alzheimer’s disease
2020
Abstract Alzheimer’s disease, being a major societal burden, demands improvement of current techniques for its treatment and diagnostics. Currently only autopsy histology is able to provide the definite diagnosis for Alzheimer’s disease. However, the procedure is rather time consuming and costly. In the current study, we utilized Stokes and Mueller polarimetry techniques to screen for amyloid-β (Aβ) deposits in formalin-fixed, paraffin-embedded mouse brain tissue at different stages of Alzheimer’s disease. The study has shown that the presence of Aβ plaques influences the properties of scattered polarized light. The Poincaré sphere was used as a graphical tool for the visualization of the a…
Polarization-sensitive hyperspectral imaging of human skin: From system design to clinical validation (Conference Presentation)
2020
We present the development and validation of a new approach for quantitative functional imaging of human skin based on the machine learning technique for the analysis of the hyperspectral skin images. The considered skin parameters include blood volume fraction, blood oxygenation, melanin content, and the epidermal layer thickness. Additionally, the degree of residual polarization of the reflected light has been analyzed. The validity of the approach has been confirmed by the initial preclinical tests with the tissue-mimicking phantoms, functional in-vivo skin tests, and pilot clinical study of type II diabetic patients. The proposed technique has great potential to be implemented in clinic…