Authentication of phacelia honeys (Phacelia tanacetifolia) based on a combination of HPLC and HPTLC analyses as well as spectrophotometric measurements
Abstract The growing interest in the quality of honey affects customer preferences and consumption trends. Generally, monofloral honeys are more expensive than multifloral honeys, and the price strictly depends on its botanical origin. The increasing popularity of a variety of monofloral brands has led to the increasing number of adulterations and therefore for the need to develop new analytical methods for assessing honey authenticity. The purpose of the present study was to develop a method for the authentication of phacelia honeys on the basis of HPLC and HPTLC analyses and spectrophotometry. The results obtained by spectrophotometric analysis indicated significant differences only betwe…
HPTLC fingerprinting—rapid method for the differentiation of honeys of different botanical origin based on the composition of the lipophilic fractions
Bee honey possess various nutritional and medicinal functions, which are the result of its diverse chemical composition. The numerous bioactive compounds in honey come from flower nectar
Botanical Origin Authentication of Polish Phacelia Honey Using the Combination of Volatile Fraction Profiling by HS-SPME and Lipophilic Fraction Profiling by HPTLC
Eleven samples of Polish Phacelia tanacetifolia Benth., three Brassica napus and one Salix spp. honeys were characterized by melissopalynology and analysis of the compositions of their volatile fractions. Headspace solid-phase microextraction coupled with gas chromatography mass spectrometry (HS-SPME/GC–MS) using PDMS/CAR/DVB fiber was used for the isolation of low-molecular weight compounds which create a volatile fraction. To differentiate and indicate the most representative unifloral samples, chemometric techniques such as principal component analysis (PCA) and hierarchical-tree clustering (HTC) were applied to the dataset of the chromatographic fingerprints. Based on the obtained resul…