Assessment of genetic variation in SicilianHelichrysum(Asteraceae) and implication to germplasm conservation

Some taxa of Sicilian Helichrysum related to sect. Stoeachadina have an uncertain systematic position; moreover, they are rare and endangered. In order to preserve these taxa in a genebank, a strategy to keep their genetic variation as high as possible is required. The high informative content of AFLP markers was used to assess genetic variations within and between populations and their relationships. The results show that all populations are genetically similar, even though there are two distinct groups: the first is the population referred to H. pendulum, and the second is represented by other populations belonging to several different specific and intraspecific taxa. The H. pendulum popu…

Relazione tra massa e qualità del seme in Raphanus sativus L.

Differences in the activity and distribution of peroxidase from three different portions of germinative Brassica oleracea seeds.

Peroxidase (POD, EC 1.11.1.7) activity, cellular localization and isozyme patterns were investigated in the seed integument, cotyledon and embryo axis of Brassica oleracea cv. Cappuccio during pregermination and seedling growth. Seeds started to germinate after 24 h of imbibition. POD activity was localized in the pigmented layer of the integument and in procambial strands of the cotyledon and embryo axis in the first 24 h of imbibition. It was localized in the integumental cells of palisade, pigmented and aleurone layers and in epidermal, meristematic, procambial cells and xylem elements of the root and hypocotyl after 48 h of imbibition. POD activity increased during germination and early…

The conservation of the endemic Crop Wild Relatives in the RIBES seed-banks: towards a national priority list for the Italian CWRs

Data of 620 crop wild relatives of species of food and fodder interest listed in the Annex I of the FAO Treaty (FAO, 2001) were collected within the census carried out by ISPRA, the Italian National Institute for Environmental Protection and Research, in 2014-2015 in order to quantify the extent of CWR representation in the Italian ex situ collections. According to such census, the RIBES network preserve 37% of the Italian CWRs listed in the FAO Treaty: in 14 out of 16 seed-banks are preserved 6,029 accessions of 229 CWR taxa, belonging to 11 families and 57 genera.

Conservation of forest biodiversity in Sicily: contribution of the University of Palermo Germplasm Bank (Italy)

Germplasm Banks dedicated to the wild plants seeds conservation play a unique role in integrating the in situ conservation of endemic, rare or endangered plant species. One of the missions of the Seed Bank of the University of Palermo (HBP-Bank) is to support the conservation of biodiversity with particular attention to the spectrum of genetic diversity of plants belonging to Mediterranean habitat. Abies nebrodensis (Lojac.) Mattei is an relict species, consisted of 30 natural individuals, gathered in a small area of the Madonie Natural Park in Sicily (1500 -1600 m above s.l.), recorded on the checklist “TOP 50 Mediterranean Island Plants”. It is also one of eight conifers species criticall…

Dormienza e germinazione in popolazioni di Ferula communis (Umbelliferae)

Genetic diversity in Sicilian Population of Quercus ilex (Fagaceae)

Evaluation of the DNA barcoding approach to develop a reference data-set for the threatened flora of Sicily

The Mediterranean Basin is one of the most significantly altered World Biodiversity Hotspots with extensive habitat loss and fast genetic population erosion, for which urgent biodiversity reconnaissance and preservation actions are required. In particular, Sicily has about 600 taxa classified as threatened or near-threatened. The correct recognition and identification of such biodiversity is required for supporting further activities. The objective of this work is to assess the ability of the DNA barcoding approach to identify different taxonomic groups from a collection of the most threatened plant taxa, throughout natural Sicilian populations. The evaluation of the DNA barcoding core mark…