0000000000311588

AUTHOR

J Buschko

showing 3 related works from this author

Anti-Idiotypic Antibodies Against the Kinin Receptor

1992

Three sets of monoclonal antibodies against bradykinin (MBK1, MBK2, and MBK3) were generated by somatic cell fusion, characterized by their peptide specificity, and compared with the known ligand specificity of the kinin receptor subtypes. By these criteria, the paratope of MBK3 resembled the B 2 receptor binding site, whereas MBK1 shared principal binding characteristics with the B 1 receptor. Anti-idiotypic antibodies against MBK1, MBK2, and MBK3 were raised in rabbit and sheep

PharmacologyIdiotypemedicine.drug_classKininBiologyLigand (biochemistry)Monoclonal antibodyMolecular biologymedicineEnzyme-linked receptorParatopeBradykinin receptorCardiology and Cardiovascular MedicineReceptorJournal of Cardiovascular Pharmacology
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Anti-Idiotypic Antibodies Against the Kinin Receptor

1992

Three sets of monoclonal antibodies against bradykinin (MBK1, MBK2, MBK3) were generated by somatic cell fusion, characterized by their peptide specificity and compared to the known ligand specificity of the kinin receptor subtypes. By these criteria the paratope of MBK3 resembled the B2 receptor binding site whereas MBK1 shared principal binding characteristics with the B1 recrptor. Anti-idiotypic antibodies against MBK1, MBK2 and MBK3 were raised in rabbit and sheep. Specificity of the network components was verified by inhibition experiments on the level of peptide, idiotype and anti-idiotype. Anti-idiotypic antibodies against MBK3 recognized a conformation-dependent epitope which was bi…

Idiotypebiologymedicine.drug_classChemistryKininMonoclonal antibodyLigand (biochemistry)Molecular biologyEpitopemedicinebiology.proteinParatopeAntibodyBinding site
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Structural features of the human bradykinin B2 receptor probed by agonists, antagonists, and anti-idiotypic antibodies

1993

The human bradykinin B2 receptor belongs to the family of G-protein-coupled receptors. To characterize the receptor protein, we have solubilized the membranes of cultured human foreskin fibroblasts bearing the B2 receptor. Affinity cross-linking of the solubilized receptor with the labeled agonist, 125I-Tyr0-bradykinin, or the labeled antagonist, 125I-(4-hydroxy-phenyl-propionyl)-HOE140, revealed major bands of apparent molecular mass of 69 kDa in SDS-polyacrylamide gel electrophoresis under reducing conditions, and of 59 kDa under non-reducing conditions. A 1000-fold molar excess of each of the unlabeled ligands quenched the specific labeling suggesting that the agonist and the antagonist …

Gel electrophoresisAgonistmedicine.drug_classChemistryInsulin-like growth factor 2 receptorCell BiologyBiochemistryMolecular biologyBiochemistrymedicineBradykinin receptorBinding siteReceptorMolecular BiologyProtease-activated receptor 2Cation-dependent mannose-6-phosphate receptorJournal of Biological Chemistry
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