0000000000324527

AUTHOR

Janina Seibert

showing 4 related works from this author

Role of en and novel interactions between msh, ind, and vnd in dorsoventral patterning of the Drosophila brain and ventral nerve cord

2010

AbstractSubdivision of the neuroectoderm into discrete gene expression domains is essential for the correct specification of neural stem cells (neuroblasts) during central nervous system development. Here, we extend our knowledge on dorsoventral (DV) patterning of the Drosophila brain and uncover novel genetic interactions that control expression of the evolutionary conserved homeobox genes ventral nervous system defective (vnd), intermediate neuroblasts defective (ind), and muscle segment homeobox (msh). We show that cross-repression between Ind and Msh stabilizes the border between intermediate and dorsal tritocerebrum and deutocerebrum, and that both transcription factors are competent t…

Neuroectodermal regionalizationNervous systemengrailedEmbryo Nonmammaliananimal structuresCentral nervous systemDorsoventral (DV) patterningBiologyNeuroblastAnterior Horn CellsmedicineAnimalsDrosophila Proteinsmsh/MsxMolecular Biologyind/GsxBody PatterningHomeodomain ProteinsVentral nerve cord (VNC)GeneticsNeuroectodermBrainvnd/Nkx2Cell BiologyBrain developmentengrailedNeural stem cellCell biologymedicine.anatomical_structureVentral nerve cordHomeoboxDrosophilaTranscription FactorsStem cell specificationDevelopmental BiologyDevelopmental Biology
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Ems and Nkx6 are central regulators in dorsoventral patterning of the Drosophila brain

2009

In central nervous system development, the identity of neural stem cells (neuroblasts) critically depends on the precise spatial patterning of the neuroectoderm in the dorsoventral (DV) axis. Here, we uncover a novel gene regulatory network underlying DV patterning in the Drosophila brain, and show that the cephalic gap gene empty spiracles (ems) and the Nk6 homeobox gene (Nkx6) encode key regulators. The regulatory network implicates novel interactions between these and the evolutionarily conserved homeobox genes ventral nervous system defective (vnd), intermediate neuroblasts defective (ind) and muscle segment homeobox (msh). We show that Msh cross-repressively interacts with Nkx6 to sust…

Nervous systemEmbryo Nonmammaliananimal structuresBiologyNeuroblastmedicineAnimalsDrosophila ProteinsMolecular BiologyGap geneBody PatterningHomeodomain ProteinsGeneticsRegulation of gene expressionNeuroectodermNeural tubeBrainGene Expression Regulation DevelopmentalCell biologymedicine.anatomical_structureVentral nerve cordembryonic structuresHomeoboxDrosophilaTranscription FactorsDevelopmental BiologyDevelopment
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Segment-specific requirements for dorsoventral patterning genes during early brain development in Drosophila.

2006

An initial step in the development of the Drosophila central nervous system is the delamination of a stereotype population of neural stem cells (neuroblasts, NBs) from the neuroectoderm. Expression of the columnar genes ventral nervous system defective (vnd), intermediate neuroblasts defective (ind) and muscle segment homeobox (msh) subdivides the truncal neuroectoderm(primordium of the ventral nerve cord) into a ventral, intermediate and dorsal longitudinal domain, and has been shown to play a key role in the formation and/or specification of corresponding NBs. In the procephalic neuroectoderm(pNE, primordium of the brain), expression of columnar genes is highly complex and dynamic, and th…

Nervous systemanimal structuresCentral nervous systemPopulationBiologyNeuroblastmedicineAnimalsDrosophila ProteinseducationMolecular BiologyIn Situ HybridizationBody PatterningHomeodomain Proteinseducation.field_of_studyNeuroectodermBrainGene Expression Regulation DevelopmentalAnatomyNeural stem cellCell biologymedicine.anatomical_structureDrosophila melanogasterVentral nerve cordembryonic structuresHomeoboxNeurogliaDevelopmental BiologyTranscription FactorsDevelopment (Cambridge, England)
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Programmed cell death in the embryonic central nervous system of Drosophila melanogaster.

2006

Although programmed cell death (PCD) plays a crucial role throughout Drosophila CNS development, its pattern and incidence remain largely uninvestigated. We provide here a detailed analysis of the occurrence of PCD in the embryonic ventral nerve cord (VNC). We traced the spatio-temporal pattern of PCD and compared the appearance of, and total cell numbers in,thoracic and abdominal neuromeres of wild-type and PCD-deficient H99mutant embryos. Furthermore, we have examined the clonal origin and fate of superfluous cells in H99 mutants by DiI labeling almost all neuroblasts, with special attention to segment-specific differences within the individually identified neuroblast lineages. Our data r…

Central Nervous SystemProgrammed cell deathanimal structuresEmbryo NonmammalianApoptosisCell CountBiologyNeuroblastInterneuronsmedicineAnimalsCell LineageMolecular BiologyBody PatterningNeuronsGene Expression Regulation DevelopmentalAnatomyNeuromerebiology.organism_classificationEmbryonic stem cellImmunohistochemistryCell biologyClone Cellsmedicine.anatomical_structureDrosophila melanogasternervous systemVentral nerve cordMutationNeuronDrosophila melanogasterGanglion mother cellDevelopmental BiologyDevelopment (Cambridge, England)
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