0000000000331828

AUTHOR

Marc Schmitz

showing 3 related works from this author

The human autoantigen La/SS-B accelerates herpes simplex virus type 1 replication in transfected mouse 3T3 cells.

1998

SUMMARY Permanently transfected mouse cell lines which expressed different levels of the human autoantigen La/SS-B were infected with different strains of herpes simplex virus type 1, including the strains ANG, HSZP, 17syn+ and HFEM. During infection the localization of the human La protein was followed using an anti-La MoAb, which recognized only the human La protein but did not cross-react with either the endogenous mouse La protein or any viral encoded protein. After infection La protein was transported from the nucleus to the cytoplasm. The time course of translocation was dependent on the amount of human La protein expressed in the respective cell line. Moreover, acceleration of viral …

virusesImmunologyHerpesvirus 1 Humanmedicine.disease_causeTransfectionVirus ReplicationAutoantigensVirus3T3 cellsSingle-stranded binding proteinMicemedicineImmunology and AllergyAnimalsHumansbiologyTransfection3T3 CellsOriginal ArticlesHerpes simplex virusmedicine.anatomical_structureViral replicationGene Expression RegulationRibonucleoproteinsCytoplasmCell cultureImmunologybiology.proteinClinical and experimental immunology
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Analysis of Epitope Spreading over an Eleven-year Period in a Patient with Systemic Lupus Erythematosus: CASE REPORT

1998

During a period of more than eleven years serum samples of a patient with Systemic Lupus Erythematosus were collected and analyzed for anti-nuclear autoantibodies. High titer of anti-La/SS-B were detectable in all serum samples. The La/SS-B epitopes remained constant. Besides anti-La/SS-B antibodies all serum samples contained traces of anti-Ro/SS-A including anti-Ro52 and anti-Ro60 antibodies. During disease flares anti-Ro/SS A antibodies were upregulated and anti-dsDNA antibodies appeared, thus supporting the concept of an antigen driven intermolecular epitope spreading to Ro/SS-A and dsDNA.

Systemic diseaseLupus erythematosusAnti-nuclear antibodybiologybusiness.industryImmunologyGeneral Medicinemedicine.diseasemedicine.disease_causeEpitopeAutoimmunityEpitope mappingRheumatologyAntigenImmunologymedicinebiology.proteinImmunology and AllergyAntibodyskin and connective tissue diseasesbusinessScandinavian Journal of Rheumatology
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Characterization of murine monoclonal antibodies against the Ro52 autoantigen

1997

SUMMARY Immunization of BALB/c mice with purified recombinant human Ro52 protein resulted in three anti-Ro52 MoAbs termed 2E7, 4C6 and 4F11. All anti-Ro52 MoAbs specifically reacted with recombinant human Ro52 protein, and also with Ro52 protein in total extracts of all human cell lines analysed, including the epithelial cell line HeLa, the B cell line Raji, the bladder carcinoma cell line RT112, and a fibroblast cell line derived from patients with xeroderma pigmentosum. The anti-Ro52 MoAbs were able to immunoprecipitate the recombinant human Ro52 protein expressed in wheat germ extract, but failed to precipitate hY RNAs from cell extracts. The staining pattern of the MoAbs strongly differ…

Editorial Reviewmedicine.drug_classMolecular Sequence DataImmunologyCellMonoclonal antibodyAutoantigensEpitopelaw.inventionHeLaMiceAntibody SpecificitylawRNA Small CytoplasmicmedicineAnimalsHumansImmunology and AllergyAmino Acid SequencebiologyAntibodies Monoclonalbiology.organism_classificationMolecular biologyRecombinant ProteinsStainingEpitope mappingmedicine.anatomical_structureRibonucleoproteinsImmunologybiology.proteinRecombinant DNAAntibodySequence AlignmentEpitope MappingGene DeletionClinical and Experimental Immunology
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