0000000000335358
AUTHOR
G. Rittner
Application of DNA Polymorphisms in Paternity Testing in Germany: Solution of an Incest Case Using Bacteriophage M13 Hybridization with Hypervariable Minisatellite DNA
More than 25 blood, serum, and enzyme polymorphisms have been introduced into paternity testing in Germany in recent years (Rittner, 1975). If a “no” decision is defined by exclusion, and a “yes” decision requires a probability of 99.73 % or more, more than 90 % of court cases can be solved in this respect. A few cases not being clarified by a standard expertise include: 1) Cases with more than one alleged man if the men and/or the mother and the men are related. 2) Some cases where the putative father is deceased, and neither the parents nor the legitimate offspring are available for the study. 3) Cases where possible exclusion in a given polymorphic system interferes with an overall evide…
Microarray mRNA expression analysis of Fanconi anemia fibroblasts.
Fanconi anemia (FA) cells are generally hypersensitive to DNA cross-linking agents, implying that mutations in the different <i>FANC</i> genes cause a similar DNA repair defect(s). By using a customized cDNA microarray chip for DNA repair- and cell cycle-associated genes, we identified three genes, cathepsin B (<i>CTSB</i>), glutaredoxin (<i>GLRX</i>), and polo-like kinase 2 (<i>PLK2</i>), that were misregulated in untreated primary fibroblasts from three unrelated FA-D2 patients, compared to six controls. Quantitative real-time RT PCR was used to validate these results and to study possible molecular links between FA-D2 and other FA subtypes.…
Minisatellite DNA Probe MZ 1.3: Application in Paternity Testing and Estimate of the Number of Genetic Loci
The use of hypervariable DNA minisatellite probes recognizing repetitive genomic DNA sequences has become a valuable and powerful tool in paternity testing as well as in forensic stain analysis (Jeffreys et al. 1985, 1987; Werrett et al. 1988). It has been shown that bacteriophage Ml3 DNA can also be used to obtain hypervariable DNA restriction fragment patterns in humans and other species (Vassart et al. 1987). To obtain more informative and specific fragment patterns for the DNA ‘fingerprint’ analysis in man, we have used Ml3 DNA as a probe to screen a human genomic library. Thus, we have isolated the minisatellite DNA probe MZ 1.3 (Schacker et al., in press). MZ 1.3 is a 1.9 kb fragment …