0000000000336759

AUTHOR

Sandra Hering

showing 3 related works from this author

Cell line DNA typing in forensic genetics—the necessity of reliable standards

2003

The incorporation of reference DNA is crucial to the validation of any DNA typing protocol. This paper aims to provide a panel of reference DNAs for actual forensic profiling strategies, i.e. autosomal and gonosomal STR typing as well as mtDNA sequencing. We have characterised three human lymphoid cell lines, GM9947, GM9948 and GM3657, and considered 58 autosomal and gonosomal microsatellites as well as the mitochondrial control region sequence. Well-established markers and STRs recently developed for forensic use were involved. K562 DNA samples which we purchased from two different suppliers were also analysed. They revealed conflicting results with regard to the ChrX STR marker genotype. …

MaleQuality ControlmtDNA control regionGeneticsMitochondrial DNAAutosomeGenotypeReference StandardsBiologyDNA FingerprintingDNA MitochondrialPathology and Forensic MedicineDNA profilingTandem Repeat SequencesCell Line TumorForensic profilingGenotypeHumansMicrosatelliteFemaleTypingK562 CellsLawDNA PrimersForensic Science International
researchProduct

New alleles and mutational events at 14 STR loci from different German populations.

2007

The molecular origin of DNA mutations and the mutation rates were analyzed at 14 short tandem repeat (STR) loci with samples from trio cases derived from 10 different German population samples. STR loci comprised of D2S1360, D3S1744, D4S2366, D5S2500, D6S474, D7S1517, D8S1132, D10S2325, D12S391, D18S51, D19S246, D20S480, D21S226, and D22S689. In a total of 488 meioses, 16 isolated genetic inconsistencies in 8 different STRs were observed, whereas no mutations were found at the other loci. The data of five mutations suggested the presence of silent or null alleles due to sequence variation in primer binding site. This could be confirmed for four suspected cases by the use of alternative prim…

GeneticsForensic GeneticsMaleMutation rateBase SequenceSTR multiplex systemDNABiologyNull allelePathology and Forensic MedicineGenetics PopulationSTR analysisGene FrequencyGermanyMutationGeneticsMicrosatelliteHumansFemalePrimer (molecular biology)AllelePrimer binding siteAllelesDNA PrimersMicrosatellite RepeatsForensic science international. Genetics
researchProduct

Mitochondrial DNA in the central european population

2000

Sequencing of mtDNA is an advanced method for the individualisation of traces. Disadvantages of this method are expensive and time-consuming analysis and evaluation procedures as well as the necessary stock of population-genetic data which is still insufficient. Central European institutes of forensic medicine from Germany, Austria, and Switzerland have been working together since the beginning of 1998 to establish a mtDNA database. The aim is to build up a large stock of forensically established data and provide population-genetic data for frequency investigations, which will serve as a basis for expert opinions and scientific research. Good data quality is ensured by using original sequen…

Mitochondrial DNADatabaseForensic anthropologyEuropean populationBiologycomputer.software_genrePathology and Forensic MedicineD-loopDNA profilingData qualityAsian countryPairwise comparisonLawcomputerForensic Science International
researchProduct