6533b7d9fe1ef96bd126c232

RESEARCH PRODUCT

Cell line DNA typing in forensic genetics—the necessity of reliable standards

Peter WiegandJeanett EdelmannLutz RoewerMatthias MichaelH. WittigInes PlateValentino RomanoReinhard SziborSandra HeringFrancesco Calì

subject

MaleQuality ControlmtDNA control regionGeneticsMitochondrial DNAAutosomeGenotypeReference StandardsBiologyDNA FingerprintingDNA MitochondrialPathology and Forensic MedicineDNA profilingTandem Repeat SequencesCell Line TumorForensic profilingGenotypeHumansMicrosatelliteFemaleTypingK562 CellsLawDNA Primers

description

The incorporation of reference DNA is crucial to the validation of any DNA typing protocol. This paper aims to provide a panel of reference DNAs for actual forensic profiling strategies, i.e. autosomal and gonosomal STR typing as well as mtDNA sequencing. We have characterised three human lymphoid cell lines, GM9947, GM9948 and GM3657, and considered 58 autosomal and gonosomal microsatellites as well as the mitochondrial control region sequence. Well-established markers and STRs recently developed for forensic use were involved. K562 DNA samples which we purchased from two different suppliers were also analysed. They revealed conflicting results with regard to the ChrX STR marker genotype. Hence, we suggest that K562 is no longer used for the calibration of profiling techniques. Our investigation establishes a panel of one female and two male DNA samples as an STR allelic ladder calibration tool and offers information on six alleles of each autosome (AS) marker, three alleles of each X chromosome (ChrX) marker and two alleles of each ChrY marker. In addition, sequences of the mitochondrial control region of the three DNAs are communicated in order to provide sequencing quality control.

yearjournalcountryeditionlanguage
2003-12-03Forensic Science International
https://doi.org/10.1016/j.forsciint.2003.09.002