0000000000337302
AUTHOR
Marta Rodriguez-alvarez
Nucleotide excision repair of abasic DNA lesions
AbstractApurinic/apyrimidinic (AP) sites are a class of highly mutagenic and toxic DNA lesions arising in the genome from a number of exogenous and endogenous sources. Repair of AP lesions takes place predominantly by the base excision pathway (BER). However, among chemically heterogeneous AP lesions formed in DNA, some are resistant to the endonuclease APE1 and thus refractory to BER. Here, we employed two types of reporter constructs accommodating synthetic APE1-resistant AP lesions to investigate the auxiliary repair mechanisms in human cells. By combined analyses of recovery of the transcription rate and suppression of transcriptional mutagenesis at specifically positioned AP lesions, w…
EGFP Reporters for Direct and Sensitive Detection of Mutagenic Bypass of DNA Lesions
The sustainment of replication and transcription of damaged DNA is essential for cell survival under genotoxic stress