0000000000345329
AUTHOR
J. Labenz
Inhibition of the herpes simplex virus-coded thymidine kinase-complex by 9-?-D-arabinofuranosyladenine 5?-monophosphate (ara-AMP) and 9-(2-hydroxyethoxymethyl)guanine-monophosphate (acyclo-GMP)
The thymidine kinase-complex isolated from herpes simplex virus type 1 and type 2 (HSV-1 and HSV-2) is associated with the following enzyme activities: ATP:dThd (dCyd) deoxypyrimidine kinase, ATP:dTMP thymidylate kinase, ADP:dThd- and AMP:dThd5′-phosphotransferase. In kinetic experiments it is shown that ara-AMP inhibits AMP:dThd- and ADP:dThd phosphotransferase activity, while acyclo-GMP impairs ADP:dThd phosphotransferase reaction only; the inhibition was found to be non-compertitive. The functional subunit ATP:dThd kinase was not affected by either compound.
Analysis of the TK enzyme complex induced by HSV types 1 and 2 by means of isoelectric focusing and polyacrylamide gel electrophoresis.
Recently we have described that the Herpes simplex virus (HSV)-induced thymidine kinase (TK) induces AMP- and ADP-dThd-5'-phosphotransferase activities. We now demonstrate the heterogeneity of the described activities in isoelectric focusing experiments and polyacrylamide gel electrophoresis. A TK--mutant of HSV type 1 fails to induce these activities. The activities of the type 1 enzyme complex was neutralized by an anti-HSV-serum. The TK-enzyme complex expressed in LTK--cells transformed to a TK+-phenotype by sheared HSV-1 DNA was compared with the wild type TK complex in isoelectric focusing experiments. Additionally we demonstrate that the HSV type 1 enzyme complex has thymidylate kinas…
Diagnostic Accuracy of <sup>13</sup>C-Urea Breath Test in the Diagnosis of <i>Helicobacter pylori</i> Infection in Patients with Partial Gastric Resection due to Peptic Ulcer Disease
<i>Aims:</i> We investigated the diagnostic properties of the <sup>13</sup>C-urea breath test (<sup>13</sup>C-UBT) prospectively. These are well validated in nonresected patients before and after treatment of <i>Helicobacter pylori</i> infection but not in patients with partial gastric resection due to peptic ulcer disease. <i>Methods:</i> Hospitalized patients with previous gastric resection and indications for upper gastrointestinal endoscopy were recruited for the study. Biopsy specimens were obtained from the fundus mucosa and the gastric remnant adjacent to the gastroenteric anastomosis for histological examination and rapid u…
Tu1253 How Safe Is Sedation in Gastrointestinal Endoscopy? a Prospective Multicenter Trial: an Interim Analysis of 73,941 Endoscopies. Data From Prospective Registries of Complications Managed by Members of the Working Group of Leading Hospital Gastroenterologists (Algk)
were those plausibly associated with the procedure and severe enough for the patient to seek medical attention. We compared the proportion of patients with adverse events associated with gastrointestinal endoscopy identified with direct patient contact to the proportion identified through our current infrastructure using the chi-square statistic. Results: During the study period, 11,710 endoscopic procedures were performed on 9,683 patients. Our study personnel made 3205 calls over a three month period and ultimately made contact with 1999 (17%; 84.5% of those called) patients. 28 (1.4%) adverse events were identified through direct patient contact. Our adverse event tracking infrastructure…