6533b7d9fe1ef96bd126cf20

RESEARCH PRODUCT

Inhibition of the herpes simplex virus-coded thymidine kinase-complex by 9-?-D-arabinofuranosyladenine 5?-monophosphate (ara-AMP) and 9-(2-hydroxyethoxymethyl)guanine-monophosphate (acyclo-GMP)

Dietrich FalkeW. E. G. MüllerJ. Labenz

subject

chemistry.chemical_classificationArabinonucleotidesGuanineKinaseAcyclovirGeneral MedicineBiologyThymidine KinaseThymidylate kinaseVirologyMolecular biologyPhosphotransferaseKineticschemistry.chemical_compoundEnzymechemistryBiochemistryMultienzyme ComplexesThymidine kinaseVirologySimplexvirusNucleotideThymidineVidarabine Phosphate

description

The thymidine kinase-complex isolated from herpes simplex virus type 1 and type 2 (HSV-1 and HSV-2) is associated with the following enzyme activities: ATP:dThd (dCyd) deoxypyrimidine kinase, ATP:dTMP thymidylate kinase, ADP:dThd- and AMP:dThd5′-phosphotransferase. In kinetic experiments it is shown that ara-AMP inhibits AMP:dThd- and ADP:dThd phosphotransferase activity, while acyclo-GMP impairs ADP:dThd phosphotransferase reaction only; the inhibition was found to be non-compertitive. The functional subunit ATP:dThd kinase was not affected by either compound.

yearjournalcountryeditionlanguage
1984-09-01Archives of Virology
https://doi.org/10.1007/bf01309993