Anhydrobiosis in yeast: Glutathione overproduction improves resistance to dehydration of a recombinant Ogataea (Hansenula) polymorpha strain
Abstract We show for the first time that a recombinant strain of yeast Ogataea (Hansenula) polymorpha is at least as tolerant to dehydration-rehydration treatment as the wild type strain. It is believed that this unusual characteristic of this recombinant yeast strain is linked with its ability to overproduce glutathione. Based on plasma membrane permeability analysis, we hypothesise that glutathione, in addition to its powerful antioxidative protective effects on membrane lipids, may also protect membrane proteins and/or nucleic acids. The combination of yeast cell dehydration with immobilisation and subsequent preliminary slow rehydration in water vapour gave good results in terms of reco…
Anhydrobiosis in yeasts: Glutathione synthesis by yeast Ogataea (Hansenula) polymorpha cells after their dehydration-rehydration.
The possibility of using active dry microbial preparations in biotechnological processes is essential for the development of new modern industrial technologies. In this study, we show the possibility of obtaining such preparations of the genetically engineered yeast strain Ogataea (Hansenula) polymorpha with glutathione overproduction. Special pre-treatment involving the gradual rehydration of dry cells in water vapour led to the restoration/reactivation of almost 100% of dehydrated cells. Furthermore, dry cells do not lose their viability during storage at room temperatures. Application of dry cells as the inoculum provides the same levels of glutathione synthesis as that of a native yeast…
Autophagy
Klionsky, Daniel J. et al.
Engineering of sugar transporters for improvement of xylose utilization during high-temperature alcoholic fermentation in Ogataea polymorpha yeast
Abstract Background Xylose transport is one of the bottlenecks in the conversion of lignocellulosic biomass to ethanol. Xylose consumption by the wild-type strains of xylose-utilizing yeasts occurs once glucose is depleted resulting in a long fermentation process and overall slow and incomplete conversion of sugars liberated from lignocellulosic hydrolysates. Therefore, the engineering of endogenous transporters for the facilitation of glucose-xylose co-consumption is an important prerequisite for efficient ethanol production from lignocellulosic hydrolysates. Results In this study, several engineering approaches formerly used for the low-affinity glucose transporters in Saccharomyces cerev…
Evaluation of the enhanced resistance of Ogataea (Hansenula) polymorpha to benzalkonium chloride as a resource for bioremediation technologies
Abstract Benzalkonium chloride (BAC) is highly abundant in wastewaters due to its wide application in household and industrial products. The aim of this research was to compare the resistance of two genetically related strains, Ogataea polymorpha (wild type) and O. polymorpha cat8Δ (recombinant strain), to enhanced concentrations of BAC (≥ 100 mg/L) in terms of its appearance in wastewaters. Inhibition of the respiration activity of wt and cat8Δ by 100 mg/L and 300 mg/L BAC in a xylose-containing broth (4 g/L xylose) was dependent on the carbon source used for obtaining the yeast biomass through ethanol fermentation. The respiration activity of wt in a liquid broth was inhibited by BAC, whi…
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
Seuls les 100 premiers auteurs dont les auteurs INRA ont été entrés dans la notice. La liste complète des auteurs et de leurs affiliations est accessible sur la publication.; International audience; In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues…
Erratum
Author(s): Klionsky, DJ; Abdelmohsen, K; Abe, A; Abedin, MJ; Abeliovich, H; Arozena, AA; Adachi, H; Adams, CM; Adams, PD; Adeli, K; Adhihetty, PJ; Adler, SG; Agam, G; Agarwal, R; Aghi, MK; Agnello, M; Agostinis, P; Aguilar, PV; Aguirre-Ghiso, J; Airoldi, EM; Ait-Si-Ali, S; Akematsu, T; Akporiaye, ET; Al-Rubeai, M; Albaiceta, GM; Albanese, C; Albani, D; Albert, ML; Aldudo, J; Algul, H; Alirezaei, M; Alloza, I; Almasan, A; Almonte-Beceril, M; Alnemri, ES; Alonso, C; Altan-Bonnet, N; Altieri, DC; Alvarez, S; Alvarez-Erviti, L; Alves, S; Amadoro, G; Amano, A; Amantini, C; Ambrosio, S; Amelio, I; Amer, AO; Amessou, M; Amon, A; An, Z; Anania, FA; Andersen, SU; Andley, UP; Andreadi, CK; Andrieu-Ab…