6533b826fe1ef96bd1283e93
RESEARCH PRODUCT
Anhydrobiosis in yeasts: Glutathione synthesis by yeast Ogataea (Hansenula) polymorpha cells after their dehydration-rehydration.
Linda RozenfeldeOlena O. KurylenkoJustyna RuchalaAndriy A. SibirnyAndriy A. SibirnyChuang-rung ChangAlexander RapoportRoksolana VasylyshynGalina KhroustalyovaRimantas Daugelavičiussubject
0106 biological sciences0301 basic medicineBioengineeringGlutathione synthesis01 natural sciencesApplied Microbiology and BiotechnologyGlutathione Synthase03 medical and health scienceschemistry.chemical_compound010608 biotechnologymedicineDehydrationDesiccationOverproductionCryptobiosisMicrobial ViabilityChemistryGeneral MedicineGlutathionemedicine.diseaseGlutathioneYeast030104 developmental biologyDehydration rehydrationBasic-Leucine Zipper Transcription FactorsBiochemistrySaccharomycetalesFluid TherapyGenetic EngineeringHansenula polymorphaBiotechnologydescription
The possibility of using active dry microbial preparations in biotechnological processes is essential for the development of new modern industrial technologies. In this study, we show the possibility of obtaining such preparations of the genetically engineered yeast strain Ogataea (Hansenula) polymorpha with glutathione overproduction. Special pre-treatment involving the gradual rehydration of dry cells in water vapour led to the restoration/reactivation of almost 100% of dehydrated cells. Furthermore, dry cells do not lose their viability during storage at room temperatures. Application of dry cells as the inoculum provides the same levels of glutathione synthesis as that of a native yeast culture.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2019-10-01 | Journal of biotechnology |