6533b7d9fe1ef96bd126d593
RESEARCH PRODUCT
Anhydrobiosis in yeast: Glutathione overproduction improves resistance to dehydration of a recombinant Ogataea (Hansenula) polymorpha strain
Mariana YurkivGalina KhroustalyovaAlexander RapoportDiana Kulikova-borovikovaRimantas DaugelavičiusAndriy A. SibirnyAndriy A. SibirnyChuang-rung ChangJustyna Ruchalasubject
0301 basic medicineStrain (chemistry)ChemistryMembrane lipids030106 microbiologyBioengineeringGlutathioneApplied Microbiology and BiotechnologyBiochemistryYeastlaw.invention03 medical and health scienceschemistry.chemical_compoundBiochemistryMembrane proteinlawNucleic acidRecombinant DNAOverproductiondescription
Abstract We show for the first time that a recombinant strain of yeast Ogataea (Hansenula) polymorpha is at least as tolerant to dehydration-rehydration treatment as the wild type strain. It is believed that this unusual characteristic of this recombinant yeast strain is linked with its ability to overproduce glutathione. Based on plasma membrane permeability analysis, we hypothesise that glutathione, in addition to its powerful antioxidative protective effects on membrane lipids, may also protect membrane proteins and/or nucleic acids. The combination of yeast cell dehydration with immobilisation and subsequent preliminary slow rehydration in water vapour gave good results in terms of recombinant strain cell viability in the dry state. Correspondingly, this recombinant strain can be used efficiently in the industrial production of glutathione.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-08-01 | Process Biochemistry |