0000000000372766
AUTHOR
James Teahan
Additional file 2 of 3 ′-5 ′ crosstalk contributes to transcriptional bursting
Additional file 2 Review history.
3 ′-5 ′ crosstalk contributes to transcriptional bursting
Abstract Background Transcription in mammalian cells is a complex stochastic process involving shuttling of polymerase between genes and phase-separated liquid condensates. It occurs in bursts, which results in vastly different numbers of an mRNA species in isogenic cell populations. Several factors contributing to transcriptional bursting have been identified, usually classified as intrinsic, in other words local to single genes, or extrinsic, relating to the macroscopic state of the cell. However, some possible contributors have not been explored yet. Here, we focus on processes at the 3 ′ and 5 ′ ends of a gene that enable reinitiation of transcription upon termination. Results Using Bay…
Additional file 1 of 3 ′-5 ′ crosstalk contributes to transcriptional bursting
Additional file 1 “Supporting Information to: “3’-5’ crosstalk contributes to transcriptional bursting”.
Supporting data to M. Cavallaro, et al., 3'-5' crosstalk contributes to transcriptional bursting, 2019
This repository contains supporting data to reference [1]. Please cite [1] if you find this repository useful. The data include: Flow cytometry data of HBB and HIV transgenes' expression in `.fcs` format. NanoString data for HIV expression. smFISH data for HBB and Akt1 gene expression. [1] M. Cavallaro, et al., 3'-5' interactions contribute to transcriptional bursting, bioR$\chi$iv 514174. https://doi.org/10.1101/514174