0000000000374194
AUTHOR
Jana Slemr
Determination of biogenic amines in meat by combined ion-exchange capillary gas chromatography
Abstract A procedure is described for the determination of putrescine, cadaverine and histamine in meat. Crude perchloric acid extracts were pre-separated on a weakly acidic cation exchanger and the amines quantified by capillary gas chromatography. The diamines were analysed as trifluoroacetyl derivatives and histamine was converted into N α -trifluoroacetyl-N τ -ethoxycarbonylhistamine. The accuracy of the determination of diamines was examined by a precipitation pre-separation method and by mass fragmentometric quantification. The proposed procedure allows the sensitive, sufficiently precise and highly specific determination of putrescine and cadaverine in meat.
Konzentrationsprofile von Putrescin und Cadaverin in beimpftem vakuumverpacktem Rindfleisch
VerschiedeneLactobacillus-, Staphylococcus-undStreptococcus-Stamme wurden auf ihre Fahigkeit gepruft, in vakuumverpacktem Fleisch Putrescin und Cadaverin zu bilden. Wahrend einer sechswochigen Lagerung bei 4 °C wurden die Proben in regelmasigen Abstanden sensorisch bewertet und ihr pH-Wert bestimmt. Den Amingehalt ermittelten wir mit Hilfe der Capillar-Gaschromatographie. Die Aminkonzentrationen blieben bis zum deutlichen Verderb am Ende der sechsten Woche im Bereich der Anfangswerte von etwa 0,08 mg/ 100 g.
Gas chromatographic and mass spectrometric analysis of bile acids as trifluoroacetyl-hexafluoroisopropyl and heptafluorobutyryl derivatives
Abstract The gas chromatographic retention times on QF-1 of 38 bile acids in the form of their trifluoroacetyl-hexafluoroisopropyl (TFA-HFIP), trifluoroacetyl-methyl, and heptafluorobutyryl derivatives are given. In general hexafluoroisopropyl ester trifluoroacetates proved superior with regard to simplicity of preparation, absence of artifacts, and resolution on QF-1. The main disadvantages of heptafluorobutyrates were the production of artifacts with some ketonic bile acids and the impossibility of separating any of the dihydroxy bile acids with substituents in the 3,6- and 3,7-positions. Mass spectra of TFA-HFIP derivatives were recorded with both direct and gas chromatographic inlet sys…