0000000000432822
AUTHOR
Françoise Plas Simon
Induction des mécanismes de défense des plantes : Les acquis du modèle tabac/cryptogéine
Cryptogein is a small protein secreted by the oomycete Phytophthora cryptogea, able to trigger on tobacco plants both the developmenl of an hypersensitive response and the induction of resistance against a wide range of pathogens. The deciphering of molecular events associated with this process, using tobacco as a model plant, gave significant informations about the set up of defense mechanisms. The role of plasma membrane dynamics in such a signal transduction has been evidenced. ln particular; the presence of plasma membrane microdomains concentrating signaling proteins, and the modification of the proteic content of these domains upon cryptogein treatment have been demonstrated. Moreover…
Rôle de la dynamique de la membrane plasmique dans la mise en place des réactions de défense chez le tabac
The binding of cryptogein, a plant defense elicitor secreted by an oomycete, to an unknown receptor triggers well-known biochemical events of early signal transduction at the plasma membrane of tobacco cells. One of these early events is the transient production of Reactive Oxygen Species (ROS) by an NADPH oxidase, NtrbohD. We were thus interested to analyze the dynamic of the plasma membrane during these early stages. We determined that internalization of the endocytotic marker FM4-64 is stimulated a few minutes after addition of cryptogein to tobacco BY-2 cells. This stimulation is specific to the signal transduction pathway elicited by cryptogein since a lipid transfer protein, which bin…