D.P14 In vitro cytotoxic activity against erythrocytes by ascidians hemocytes: Target/effector interactions

The cell-mediated, calcium-dependent cytotoxic activity against mammalian erythrocytes by the hemocytes of the ascidians Ciona intestinalis and Stye/a plicata was investigated by in vitro cytotoxic assay (HCA). Hemocytes/erythrocytes mixtures were performed in artificial sea water or TBS enriched with 10 mM CaC12, at 25° or 3rC, and the released haemoglobin was estimated by reading the absorbance at 541 nm (Parrinello et al., D.C.I., 17:19, 1993). With the aim to examine the cytotoxic mechanism involved, inhibition experiments with lipidic components of the erythrocyte membrane have been carried out in TBS-Ca. Among the phospholipids tested sphingomyelin is an inhibitor of the Ciona intesti…

RISPOSTA IMMUNITARIA DEL PUNTERUOLO ROSSO NEI CONFRONTI DEL ENTOMOPATOGENO BACILLUS THURINGIENSIS

Introdotto accidentalmente in Sicilia nel 2005 Rhynchophorous ferrugineus Olivier (Coleoptera Curculionidae), noto come punteruolo rosso, ha infestato velocemente e gravemente molte palme. La sua espansione è veloce e i potenziali stress abiotici e biotici presenti nel nuovo areale non sembrano costituire dei possibili agenti di contenimento. Si è cercato di comprendere il perché di tale resistenza trattando larve di punteruolo con uno dei maggiori batteri entomopageni attualmente conosciuti il Bacillus thuringiensis Berliner (Bt). Gli studi eff ettuati hanno evidenziato una certa mortalità ma soprattutto un’interazione con l’attività trofi ca. Per comprendere meglio questi eff etti si è st…

FACIT collagen (1alpha-chain) is expressed by hemocytes and epidermis during the inflammatory response of the ascidian Ciona intestinalis

Based on previous cloning and sequencing study, real-time PCR and in situ hybridization assays of the inflamed body wall of LPS-injected Ciona intestinalis showed the enhanced gene expression of a collagen with FACIT structural features (Ci-type IX-Col 1a-chain). By using specific antibodies raised against an opportunely chosen Ci-type IX-Col synthetic peptide, the fibroblast property of hemocytes challenged in vitro with LPS (at 4 h) was displayed by flow cytometry, while immunocytochemistry identified hemocytes with large granules (morula cells) as collagen-producing cells. Hemocyte lysate supernatant analyzed in immunoblotting contained a 60 kDa band identifiable as 1a-chain-Ci-type IX-C…

Localizzazione di un recettore glucocorticoideo DLGR1 nei tessuti del teleosteo Dicentrarchus labrax

Genetic Variability of Mediterranean Sea red mullet Mullus barbatus (Pisces, Mullidae)

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Purification and characterization of an f-type lectin from small-spotted catshark (Scyliorhinus canicula)serum

Preliminary evidence for the cytotoxic molecule in hemocyte supernatant lysate from Ciona intestinalis unilocular hemocytes

Galectine nella risposta infiammatoria di Ciona intestinalis cDNA ed espressione genica

Expression and distribution of DIGR1 in the ontogeny of Dicentrarchus labrax

Modulazione In vivo dell’Hsp70 negli emociti di Rhynchophorus ferrugineus dopo trattamento con Bacillus thuringiensis

Diversi tipi di stress ambientali possono indurre l’espressione delle Hsps vengono rapidamente sintetizzate all'interno delle cellule dopo esposizione ad uno stressor. Le HSPs sono raggruppate in diverse famiglie in base alle dimensioni delle proteine. Nella maggior parte degli organismi ci sono diversi geni che codificano i membri di questa famiglia HSP. In studi precedenti è stata valutata l'interazione tra Bacillus thuringiensis e gli emociti circolanti di Rhynchophorus ferrugineus il punteruolo rosso delle palme. Questo coleottero è grande, da due a cinque centimetri di lunghezza, ed è di colore rosso ruggine, le sue larve scavano buche fino a un metro lungo il tronco delle palme, e può…

Putative rhamnose-binding lectin in the solitary ascidian Ciona intestinalis.

Attività lisozima-simile e citotossica nel muco di Actina equina (Cnidaria, Anthozoa)

Marcatori genetici molecolari e caratterizzazione delle cultivar

Isolation, characterization and expression analysis of a collectin in Tunicate Ciona intestinalis.

Artificial environmental conditions can affect allozyme genetic structure of the marine gastropod Patella caerulea

Samples of the Mediterranean limpet Patella caerulea collected from 10 sites were examined for genetic population structure. Six of the 20 identified enzymatic loci were polymorphic. The AAT* locus was polymorphic only in two samples from an artificial environment (TI2 and PE). The proportion of polymorphic loci ranged from 0.20 to 0.30, and the observed and expected mean heterozygosity varied between 0.098 and 0.076 and between 0.109 and 0.086, respectively. Mean Fis values were significantly positive in AAT*, ESTD*, PEPC-2*, and PEPD*, showing heterozygosity deficiency. In all, mean Fst value of 0.007 indicated high genetic homogeneity between the samples analyzed, whereas single-locus Fs…

Isolamento, caratterizzazione e distribuzione cellulare di una neurotossina ad attività litica di Actinia equina (Anthozoa, Cnidaria

Anti biofilm activity of Paracentrotus lividus coelomocytes

Cellule che producono ciTNFalfa sono attive nell'infiammazione e nello sviluppo larvale di Ciona intestinalis

Are there any distinct genetic sub-populations of sand smelt, Atherina boyeri (Teleostei: Atherinidae) along Italian coasts? Evidence from allozyme analysis

Two hundred and ninety-nine specimens of Atherina boyeri, collected from five Mediterranean sites (three from the open sea (Chioggia, Catania and Gaeta) and two from river mouths (the Birgi and S. Bartolomeo rivers) were analyzed in order to confirm previous genetic studies. Eigtheen loci were scored from 12 enzymatic systems, 3 of which were polymorphic: EST*, PGM* and PGI*. Mean Fst value (0.300, p< 0.05) indicated a high genetic heterogeneity. Pairwise Fst and genetic distance revealed that samples from the river mouths (Birgi and S. Bartolomeo) were grouped separately from the open sea samples (Catania, Chioggia and Gaeta). These results are in agreement with the most recent classificat…

A D-galactose specific lectin is an inducible inflammatory IL-1-like opsonin in the hemolymph of the ascidian Ciona intestinalis challegend with LPS.

Attivazione del sistema della profenolossidasi nella risposta infiammatoria della tunica di Ciona intestinalis (L.)

The italian association of developmental and comparative immunology (IADCI)

Preliminary results on the interaction between Bacillus thuringensis and Red Palm Weevil.

Exosome release by crustacean hyaline haemocytes in vitro

Exosomes are small microvesicles (40–100 nm) that are formed from the endosome to generate multi-vesicular bodies (MBVs). In mammals, exosomes play a significant role in cellular communication. Exosomes have also been reported for salmon and fruit fly but have not previously been studied in crustaceans. Therefore we undertook to study them in decapods. We chose the hyaline haemocytes of brachyuran crabs, Carcinus maenas and Hyas araneus, because these cells are abundant in haemolymph, are phagocytic and can be cultured on glass or plastic surfaces. Furthermore these cells have been observed to contain MVB-like structures that bear some resemblance to those in mammals that produce exosomes. …

Attività biologica e caratterizzazione molecolare di un peptide neurotossico ad attività litica di Actinia equina (Anthozoa, Cnidaria)

Espressione dell'attività acetilcolinesterasica nelle cellule lateralidel tronco della larva e negli emociti di Ascidie solitarie

Natural immunity in Paracentrotus lividus: coelomocyte cooperation

Lectine sieriche di "tipo F" nell'immunità innata dei pesci: aspetti molecolari e funzionali.

Purification and characterization of D-galactose binding lectin involved in the inflammatory response in Ciona intestinalis

Effects of exogenous cortisol on the expression of glucocorticoid receptor (DIGR1) and hsp70 in head-kidney and peritonel cavity cells from Dicentrarchus labrax.

Further insight on Ciona intestinalis prophenoloxidase system activated during the LPS induced inflammatory response

APOPTOSIS IN ASCIDIAN LARVAE AFTER TRIBUTYLTIN-CHLORIDE TREATMENT

Effetti del cadmio sull'espressione delle HSP70 nelle cellule del sangue di Spigola (Dicentrachus labrax L. Osteichthyes, Moronidae)

A glucocorticoid receptor in sea bass leukocytes

Inflammatory responses of the ascidian Ciona intestinalis.

Serum lectins in fish innate immunity: molecular and functional aspects

Fucose-binding lectins (FBL) are present in tissues and fluids from invertebrates and vertebrates. The lectin repertoires in teleost fish are highly diversified and recently has been described the structure of the fucose-binding agglutinin that revealed a novel lectin fold (the “F-type” eel (Anguilla anguilla) fold), which shared a unique fucose-binding sequence motif contained both in carbohydrate-binding proteins and unrelated proteins. In this report, we describe serum FBL from sea bass Dicentrarchus labrax and sea bream Sparus aurata. These lectins were purified, characterized, cloned and sequenced. Studies on structural aspects, biological activity, tissue distribution as well as ontog…

Purificazione di una lectina calcio dipendente dal muco di Sabella spallanzanii (Polychaeta: Sabellidae)

First evidences of Toll like receptor on Paracentrotus livisua coelomocytes.

Caratterizzazione molecolare di un peptide antimicrobico isolato dai leucociti del teleosteo Dicentrarcus labrax

In vivo modulation of Hsp70 in Rhynchophorus ferrugineus hemocytes after Bacillus thuringiensis treatment

Heat shock proteins (Hsps) are rapidly synthesized within stressed cells after exposure to an environmental stressor. A variety of environmental stresses, including heat, cold, trace-metal exposure, xenobiotics have been reported to modulate Hsps expression in various organisms. Hsps are grouped into several families based on their protein size. Most organisms have several genes encoding members of this Hsp family. In particularly Hsp70 can be induced quickly under stressful conditions, but return to a normal expression level under non-stressful conditions. Few studies have been done to detect the Hsp70 expression in phytophagous insects towards pathogens. Since a preliminary research discl…

Morphological characterization and acetylcholinesterase activity in Ciona intestinalis hemocytes

Molecular characterization of macrophage migration inhibitory factor (MIF) from mussel, Mytilus galloprovincialis

Multi target antimicrobial peptides from mussel and the paradox af ayster.

Evoluzione del Museo Naturalistico tradizionale verso le nuove tecnologie multimediali per un'offerta formativa e culturale multivalente.

Invertebrate lectins present cytokine properties.

Inflamed adult pharynx tissues and swimming larva of Ciona intestinalis share CiTNFalfa-producing cells

Biological activity, tissue distribution and preliminary moleular characterization of a serum fucolectin from the sea bass (Dicentrarchus labrax)

Unità operativa "biotassonomia"

Nuovi dati sulla risposta infiammatoria dell’ascidia Ciona intestinalis

A response of Rhynchophorous ferrugineus (Coleoptera: Curculionidae) larval hemocytes to Bacillus thuringiensis

Assessment of inbreeding in hatchery samples of the red seabream Pagrus major (Perciformes: Sparidae).

Effetti modulanti del poliacrilato sulla tossicità del TMT-CI in immunociti di Paracentrotus lividus.

Tunicate immunocytes can be cytotoxic toward foreign cells

Sphingomyelin as well as carbohydrates are involved in the mechanism of cytotoxic molecules contained and released in vitro by Ciona intestinalis granulocytes.

Espressione e distribuzione del recettore DLGR1 nel cervello del Teleosteo Dicentrarchus labrax.

Espressione di recettori glucocorticoidei in cellule dell’immunità innata di Dicentrarchus labrax

Early developmental stage of WSSV in Marsupenaeus japonicus

Espressione e distribuzione del recettore DLGR1 nell’ontogenesi di Dicentrarchus labrax.

Il sistema della profenolossidasi in Ciona intestinalis. Caratterizzazione e interazione tra fenolossidasi, perossinectina e superossido dismutasi Cu-Zn dipendente nella risposta infiammatoria indotta da LPS

Functional differential hemocytes behaviour in the clearance of bacteria and humoral defense factors variability in Mytilus galloprovincialis

Genetic and morphometric variation of Mediterranean hake, Merluccius merluccius, in the Strait of Sicily (Central Mediterranean)

Allozyme, morphometric and growth analyses were applied to samples collected in the Strait of Sicily (central Mediterranean Sea) to test the hypothesis of the existence of a unique hake stock (Merluccius merluccius) in the study area. The level of genetic variation was detected from five polymorphic loci (ADH*, PGI-1*, PGI-2*, PGM*, SOD-1*). The average observed heterozygosity amounted to 0.421, while the average expected was 0.353. Weir & Cockerham statistics showed no heterogeneity, except for the single PGI? locus (theta = 0.011; P 0.05). Morphometric analyses revealed some differentiation. Females showed different relationships in six out of eight morphometric indices with total length,…

Sistema immunitario del Punteruolo rosso

Modulation of prophenoloxidase activity in Ciona intestinalis inflammatory response

Effetti di Bacillus thuringiensis nella sua forma vegetativa sulle larve di Rhynchophorous ferrugineus (Coleoptera Curculionidae)

Il batterio Bacillus thuringiensis (Bt) è un patogeno di molti insetti ed è ampiamente impiegato, sottoforma di spore, nella lotta biologica. La forma vegetative (Btv) è considerata quella responsabile della setticemia dell’ospite dopo che le tossine (Cry) rilasciate dal corpo parasporale del Bt in forma sporulata (Bts) hanno perforato il mesenteron del ospite. Tuttavia poco è conosciuto relativamente ai meccanismi di difesa dell’insetto nelle fasi successive alla azione delle tossine Cry. Lo scopo del presente lavoro è quello di studiare gli effetti del Btv sulle larve del fitofago Rhynchophorous ferrugineus Olivier (Coleoptera: Curculionidae) a seguito di iniezioni sub letali di Btv. Ques…

Modulation of immune gene expression by environmental conditions in mollusks.

RAPD (Random Amplified Polymorphic DNA) profiles distinguish Paracentrotus lividus populations living in a stressing environment (Amvrakikos Gulf, Greece)

Random amplified polymorphic DNA (RAPD) analysis was performed to assess genetic markers of Paracentrotus lividus populations living in stressing environment in the Amvrakikos Gulf (Western Greece, Ionian Sea) where two populations distinguishable in body size, smaller than the open sea ones, were detected. The UPGMA dendrogram, constructed from pairwise . st values among population nuclear DNA markers, revealed that the small and medium-sized populations living inside the Amvrakikos presented a lower polymorphism, and form a cluster that shows the genetic distance with normal-sized populations (Ionian and Tyrrhenian Seas) living in open sea. AMOVA analysis indicated a genetic distance amon…

The cytotoxic activity of Ciona intestinalis (ascidian) unilocular refractile hemocytes versus K562 tumor cells and mammalian erythrocytes involves phospholypase A2 and lectins

Localizzazione di una lectina di “tipo F” nell’adulto e nell’ontogenesi di Dicentrarchus labrax

The purification, cloning, sequencing, molecular properties and expression of a fucose-binding lectin from the serum of Dicentrarchus labrax (DlFBL) have been previously reported. We now describe the distribution and expression of DlFBL during fish ontogeny. Immunohistochemistry and in situ hybridization assays were carried out at various developmental stages (from 10 days posthatching larvae to juveniles). Another fucose-binding lectin, similar to DlFBL in biochemical, immunochemical and agglutinating properties, was extracted and purified from eggs and appeared to be localized in the embryo yolk sack residual. DlFBL was found in columnar and goblet cells of the intestinal epithelium of la…

Molecular cloning, characterization and expression analysis of peroxinectin from Ciona intestinalis

Stress e immunità nei gamberi allevati

Expression of a glucocorticoid receptor (D1GR1) in several tissues of the teleost fish Dicentrarchus labrax

Since glucocorticoids have a role in maintaining the homeostatic status in fish, in the present paper mRNA expression (in situ hybridization) and tissue immunohistochemical localization of a glucocorticoid receptor (DlGR1) in several Dicentrarchus labrax organs are reported. Riboprobe and specific antibodies were prepared by using the DlGR1 that has been previously cloned and sequenced from peritoneal cavity leukocytes. Both mRNA and receptor were identified in head kidney, spleen, gills, intestine, heart and liver tissues. The functional roles of DlGR1 localization are discussed.

Hemocytes and humoral defense factors variability from different Mediterranean populations of Mytilus galloprovincialis

Variabilità dei peptidi antimicrobici negli invertebrati: esempio della mitilina da Mytilus galloprovincialis

Il fattore di crescita dell'Endotelio vascolare (VEGF) e il suo recettore (VEGFR) nell'ascidia solitaria Ciona intestinalis

Expression of a type IX-like collagen in tissue injury of the ascidian Ciona intestinalis durino inflammatory process

Espressione e distribuzione del recettore DLGR1 nel cervello del teleosteo Dicentrarchus labrax (L. 1758)

Risposta allo stress ambientale di Dentex dentex, Pagrus major ed il loro ibrido “Pantice”.

Effetti modulanti del cadmio e del rame sulle attività cellulari del teleosteo Dicentrarchus labrax

Effetti tossici del metilmercurio sulle risposte immunitarie dell'ascidia Styela plicata

Il sistema della profenolossidasi nella risposta infiammatoria della tunica di Ciona intestinalis (L.)

In vitro effects of methylmercury on ascidian (Styela plicata) immunocyte responses

This study shows that high methylmercury concentrations are cytotoxic for Styela plicata hemocytes, whereas sublethal concentrations affect immunocyte responses. Moreover, hemocytes exposed to the xenobiotic present a significantly enhanced phenoloxidase activity as revealed in the hemocyte lysate supernatant compared with the control. Although the cytotoxic activity of S. plicata hemocytes toward rabbit erythrocytes is a PO-dependent cell-target reaction due to quinone products, it was significantly decreased by suitable methylmercury concentrations in the medium. The same xenobiotic concentrations decreased the hemocyte phagocytic activity toward yeast. In both the responses cell-target c…

Attività biologica, distribuzione tissutale e caratterizzazione molecolare della fucolectina sierica di spigola (Dicentrarchus labrax).

Characterization of cellular and molecular responses of Actinia equina (Linnaeus, 1758)

In vivo modulation of Bacillus thuringiensis (commercial form) on Holothuria tubulosa immune defense mechanisms

In invertebrates, cellular and humoral components are evolved to maintain their homeostais and integrity. Both these components respond to different non-self such as microorganisms, vertebrate erythrocytes and foreign proteins. The aim of this research was to study the possible modulating effects of Bacillus thuringiensis (Bt) in a commercial and vegetative form on the immune response of the echinoderm, sea cucumber Holothuria tubulosa. Bt is one of the most used biopesticides worldwide, covering the 90% of the organic market. Sicily is a region vocated to biological control and agricultural area are often closed to the coastal area. Investigate the effect of biopesticides on other non targ…

Differente ruolo delle subpopolazioni degli emociti di Mytilus galloprovincialis nella clearance batterica

An Attempt to re-examine the immune role of Ciona intestinalis hemocytes

Aspetti filogenetici della nuova famiglia di lectine "di tipo F" nei Pesci.

Attività proliferante degli emociti di Ciona intestinalis

La proliferazione è il termine che definisce il processo di crescita delle popolazioni cellulari. Nell’ematopoiesi dei Vertebrati, la cellula staminale trascorre la maggior parte del suo ciclo vitale nello stadio G0 e si divide per produrre il progenitore staminale in grado di proliferare attivamente. In Ciona intestinalis poco si sa circa l’ematopoiesi sebbene, alcuni autori hanno descritto nel faringe zone di proliferazione definite col termine di noduli emopoietici e, per gli emociti cosiddetti linfocito-like, un’attività proliferativa in seguito a stimolazione. In questo lavoro viene riportata l’attività proliferante degli emociti circolanti. Gli emociti sono stati caratterizzati utiliz…

Evoluzione dell’immunità innata. Componenti della reazione infiammatoria indotta da LPS nella parete corporea dell’ascidia Ciona intestinalis.

Molecular characterization of an antimicrobial peptide isolated from leucocytes of the teleostean Dicentrarchus labrax.

Mussel response to anthropic pressures measured by HSP70 gene expression in Q-PCR.

Espressione di galactine nell'infiammazione e nella larva natante di Ciona intestinalis

Enhanced CiTNFα expression is an inflammatory response of the ascidian Ciona intestinalis

Il sistema della profenolossidasi in Ciona intestinalis durante il processo infiammatorio indotto da LPS

The Ciona intestinalis prophenoloxidase activating system during LPS inflammatory reaction

I cordati invertebrati: tunicati e cefalocordati

Quantitative expression of antimicrobial peptide genes in mussel.

Musei e divulgazione naturalistica. Il contributo del Museo Zoologico “P. Doderlein” dell’Università di Palermo

The ascidian Ciona intestinalis as experimental model for the study of the complement system inflammatory pathway in deuterostome invertebrates

Nuovi dati sull'attivazione della profenolossidasi di Ciona intestinalis nel processo infiammatorio

Stress and immunomodulation indicators in gilthead seabream (Sparus aurata): preliminary data concerning the alterations due to the social behaviour.

Prophenoloxidase system is activated in the tunic inflammatory response of ciona intestinalis

Inflammation in ascidians

Indicatori di stress ed immunomodulazione in Sparus aurata: dati preliminari sulle modificazioni indotte dal comportamento sociale di sottomissione.

Molecules and cells in inflammatory responses of the ascidian Ciona intestinalis

Influenza della sostanza organica policarbossilica sulla tossicità di triorganostagno in ambienti marini. Effetti modulanti del poliacrilato sulla tossicità del trimetilstagno (IV) in immunociti di Paracentrotus lividus.

Variabilità genica dei peptidi antimicrobici: polimorfismo dell’mRNA della mitilina B di M. galloprovincialis

Modulazione cortisolo-dipendente delle Hsp 70 e 90 nel pronefro di Dicentrarchus labrax in vivo ed in vitro

La risposta infiammatoria in Ciona intestinalis è caratterizzata dall’aumento di lectine specifiche per D-galattosidi e dall’espressione di collagene FACIT di tipo IX

Comparative analysis of fucose binding lectins isolated and characterized from different teleost species, and distribution of a F-Lectin during Dicentrarchus labrax ontogenesis

Isolation and cytotoxic activity of neurotoxin from the mucus of Actinia equina (Anthozoa, Cnidaria).

Effetti del cadmio sulla fagocitosi e sull'attività di formazione di placche di lisi dei celomociti di Parcentrotus lividus

Modificazioni istologiche dei follicoli tiroidei dopo un trattamento con tributilstagno cloruro in esemplari giovanili di Liza saliens

Molecular and physiological characterization of in vivo Sulfamethoxazole response in Procambarus clark

Preliminare caratterizzazione di un’attività citotossica nel supernatante del lisato degli emociti con granulo rifrangente di Ciona intestinalis

Isolamento di nuovi peptidi citolitici da Actinia equina (Anthozoa, Cnidaria)

Evolution of Innate Immunity. Components of inflammatory reaction in Ciona intestinalis

Attività citotossica nel muco di Actinia equina (Anthozoa, Cnidaria)

Localization of glucocorticoid receptor DIGR1 in tissues pf teleost Dicentrarchus labrax.

Attività antibatterica e agglutinante nel muco di Sabella Spallanzanii (Annelida, Polychaeta)

A serum fucose-binding lectin (D1FBL) from adult Didentrarchus labrax is expressed in larva and juvenile tissue and contained in eggs

The purification, cloning, sequencing, molecular properties and expression of a fucose-binding lectin from the serum of Dicentrarchus labrax (DlFBL) have been previously reported. We now describe the distribution and expression of DlFBL during fish ontogeny. Immunohistochemistry and in situ hybridization assays were carried out at various developmental stages (from 10 days posthatching larvae to juveniles). Another fucose-binding lectin, similar to DlFBL in biochemical, immunochemical and agglutinating properties, was extracted and purified from eggs and appeared to be localized in the embryo yolk sack residual. DlFBL was found in columnar and goblet cells of the intestinal epithelium of la…

Espressione di DlGR1 nell'intestino di D.labrax dopo trattamento in vivo con cortisolo

Meccanismi dell’immunità naturale in echinodermi. Interazioni tra i celomociti di Paracentrotus lividus

Espression of a type IX-like collagen in tissue injury of the ascidian Ciona intestinalis during inflammatory process

INDAGINI SULLA RISPOSTA IMMUNITARIA DEL PUNTERUOLO ROSSO NEI CONFRONTI DEI NEMATODI ENTOMOPAGENI

Il Punteruolo rosso delle palme (Rhynchophorus ferrugineus Olivier, 1790. Coleoptera Curculionidae), originario dell’Asia meridionale si è velocemente diff uso nell’areale mediterraneo attaccando diversi generi di palme e creando seri problemi al settore ornamentale e vivaistico, oltre che alle aree verdi delle città. Il controllo di questo fi tofago si è rivelato problematico e di diffi cile realizzazione a causa delle sue proprietà invasive. Allo stato attuale non sono stati individuati patogeni effi caci nella lotta biologica, anche se i nematodi entomopatogeni (EPN) sono stati impiegati con discreto successo in Spagna. Generalmente gli EPN sono impiegati contro gli stadi larvali ma poic…

Dal Campo al Museo, le scienze naturali attraverso la didattica ambientale

Enhanced expression of a CinTNF gene in the LPS challenged inflammatiry responses of the ascidian Ciona intestinalis

Interazioni tra il sistema immunitario del Punteruolo rosso e il batterio entomopatogeno Bacillus thuringensis

Effects of cadmium on expression of the hsp70 in sea bass (Dicentrarchus labrax L., Osteichthyes. Moronidae) blood cells

Abstract To gain further insight into the role of cortisol in Wsh innate immune responses, we cloned and sequenced a 2592 bp cDNA from sea bass (Dicentrarchus labrax) peritoneal leukocytes (PCLs) encoding a glucocorticoid receptor (DlGR1). The deduced aminoacid sequence displayed that DlGR1 belong to a multigenic family of steroid hormone receptors, and exhibited high homology (80%) to the Burton’s mouth breeder (Haplochromis burtoni) HbGR1. The DlGR1 functional domains presented homologies with those of several vertebrate species. In situ hybridization assay revealed that DlGR1 was expressed in macrophages and neutrophils from the peritoneal cavity. Since in a previous paper, sea bass PCL …

Individual variability and gene expression specificity of the mussel antifungal mytimycin (MytM)

Inflammatory response of Ciona intestinalis involve enhanced D-galactoside-specific lectins and FACIT collagen expression.

Characterization of 2 viruses (Mr NV), pathogenic agents of the white taildisease (WTD) of the giant freshwater prawn macrobrachium rosenbergii: an anusual viral association.

Effect of cadmium exposure on phagocytosis and plaque lysis activity of Paracentrotus lividus coelomocyte

Focusing on Ciona intestinalis (Tunicata) innate immune system. Evolutionary implications

Phylogenetic analyses based on molecular data provide compelling evidence that ascidians are of critical importance for studying chordate immune system evolution. The Ciona intestinalis draft genome sequence allows searches for phylogenetic relationships, gene cloning and expression of immunorelevant molecules. Acidians lack of the pivotal components of the vertebrate recombinatory adaptive immunity, i.e., MHC, TCRs and dimeric immunoglobulins. However, bioinformatic sequence analyses recognized genic elements indicating the essential features of the Ig superfamily and ancestor proto-MHC genes, suggesting a primitive pre-duplication and pre-recombination status. C. intestinalis genes for in…

Primary structure and opsonic activity of an F-lectin from serum of the gilt head bream Sparus aurata (Pisces, Sparidae)

Cooperazione cellulare nella reazione citotossica dei celomociti di Paracentrotus lividus

Anche i pesci si stressano

Palermo, S. Rosalia e la cultura della Biodiversità