0000000000444792
AUTHOR
Mio Nakamura
Anthraquinones from Ophiorrhiza pumila tissue and cell cultures
We have succeeded in initiating and establishing systems of tissue and cell cultures of Ophiorrhiza pumila. Examination of the constituents of the methanol extract of the cultured calli revealed the presence of 11 anthraquinones including two new ones whose structures have been rigorously proved using advanced spectroscopic methods. These findings demonstrated a remarkable difference in the constituents between the wild plants and the callus tissue or cultured cells; the former is devoid of anthraquinones and contains a variety of camptothecin-related alkaloids whereas the latter contains a significant amount of anthraquinones and shows no indication of the presence of alkaloids after sever…
Constituents of regenerated plants of Ophiorrhiza pumila; formation of a new glycocamptothecin and predominant formation of (3R)-deoxypumiloside over (3S)-congener
Abstract The regeneration of plantlets was successful from Ophiorrhiza pumila callus cultures, from which a new glucosyloxy camptothecin, 9-β-glucosyloxycamptothecin, together with 15 metabolites including six camptothecin-related alkaloids was isolated. (3S)-Deoxypumiloside, one of the plausible biogenetic precursors of camptothecin, could not be found, whereas the (3R) epimer was isolated from the regenerated plants.