0000000000444814
AUTHOR
Gerd Brunner
Butyrate-Induced Growth Arrest of GH3-Cells is not Linked to a Distinct Morphological Phenotype
N-butyric acid is known to be a potent proliferation-inhibitor of a great number of cell types, both normal and neoplastic (1). In many cases growth arrest is accompanied by striking changes in morphology, e.g. formation of cell processes or increased spreading (1). These changes can be traced back to altered glycolipide and glycoprotein patterns of the plasma membrane and to a reorganization of the cytoskeleton (2, 3).
A New Culture Technique: An Approach to the in-Vitro Reconstitution of Endocrine Organs
A new cell culture technique called three-dimensional-rotation culture (3dR-culture) has been developed. Its main characteristics are: (i) cultivation under constant rotation, and (ii) the use of a three-dimensional material that allows the cells to adhere and to grow in a three-dimensional arrangement. Two glassfibre materials and one gelatine-sponge proved suitable for the use in cell culture. The new technique has the advantage to yield in large numbers cells bound on a stable matrix that can easily be handled, e.g. for implantation.