0000000000447133
AUTHOR
Diana C. F. Monteiro
Using synchrotrons and XFELs for time-resolved X-ray crystallography and solution scattering experiments on biomolecules
International audience; Time-resolved structural information is key to understand the mechanism of biological processes, such as catalysis and signalling. Recent developments in X-ray sources as well as data collection and analysis methods are making routine time-resolved X-ray crystallography and solution scattering experiments a real possibility for structural biologists. Here we review the information that can be obtained from these techniques and discuss the considerations that must be taken into account when designing a time-resolved experiment.
Photocage-initiated time-resolved solution X-ray scattering investigation of protein dimerization
Photocaging in combination with X-ray solution scattering allows for the time-resolved study of protein dynamics in solution. This method is versatile and allows for accurate triggering of protein function.