0000000000447133

AUTHOR

Diana C. F. Monteiro

showing 2 related works from this author

Using synchrotrons and XFELs for time-resolved X-ray crystallography and solution scattering experiments on biomolecules

2015

International audience; Time-resolved structural information is key to understand the mechanism of biological processes, such as catalysis and signalling. Recent developments in X-ray sources as well as data collection and analysis methods are making routine time-resolved X-ray crystallography and solution scattering experiments a real possibility for structural biologists. Here we review the information that can be obtained from these techniques and discuss the considerations that must be taken into account when designing a time-resolved experiment.

NanotechnologyElectrons010402 general chemistryCrystallography X-Ray01 natural sciencesCatalysi03 medical and health sciencesStructural BiologyScattering radiationHumansScattering RadiationStructural DynamicMolecular BiologyAnalysis method030304 developmental biologychemistry.chemical_classificationPhysics[PHYS]Physics [physics]0303 health sciencesScatteringBiomoleculeLasersReaction TriggeringSettore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)0104 chemical sciencesSolutionschemistryX-ray crystallographyAtomic physicsStructural Biology; Structural Dynamics; Catalysis; Reaction TriggeringSynchrotronsCurrent Opinion in Structural Biology
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Photocage-initiated time-resolved solution X-ray scattering investigation of protein dimerization

2018

Photocaging in combination with X-ray solution scattering allows for the time-resolved study of protein dynamics in solution. This method is versatile and allows for accurate triggering of protein function.

0301 basic medicineKineticsBiochemistryQuantitative Biology::Subcellular Processes03 medical and health sciencesProtein structurebiophysicsstructural biologyGeneral Materials SciencephotocagingProtein Dimerization[PHYS]Physics [physics]Quantitative Biology::BiomoleculesCrystallographyChemistryScatteringQuantitative Biology::Molecular NetworksX-rayGeneral ChemistryCondensed Matter PhysicsbiophysicSmall moleculeX-ray solution scatteringResearch LettersSettore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)030104 developmental biologyStructural biologyQD901-999BiophysicsIUCrJ
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