6533b81ffe1ef96bd1277bae
RESEARCH PRODUCT
Using synchrotrons and XFELs for time-resolved X-ray crystallography and solution scattering experiments on biomolecules
Arwen R. PearsonBriony A. YorkeMatteo LevantinoMarco CammarataDiana C. F. Monteirosubject
NanotechnologyElectrons010402 general chemistryCrystallography X-Ray01 natural sciencesCatalysi03 medical and health sciencesStructural BiologyScattering radiationHumansScattering RadiationStructural DynamicMolecular BiologyAnalysis method030304 developmental biologychemistry.chemical_classificationPhysics[PHYS]Physics [physics]0303 health sciencesScatteringBiomoleculeLasersReaction TriggeringSettore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)0104 chemical sciencesSolutionschemistryX-ray crystallographyAtomic physicsStructural Biology; Structural Dynamics; Catalysis; Reaction TriggeringSynchrotronsdescription
International audience; Time-resolved structural information is key to understand the mechanism of biological processes, such as catalysis and signalling. Recent developments in X-ray sources as well as data collection and analysis methods are making routine time-resolved X-ray crystallography and solution scattering experiments a real possibility for structural biologists. Here we review the information that can be obtained from these techniques and discuss the considerations that must be taken into account when designing a time-resolved experiment.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-12-01 | Current Opinion in Structural Biology |