0000000000481675

AUTHOR

I Pernice

showing 3 related works from this author

Microsatellite-based genotyping of Candida parapsilosis sensu stricto isolates reveals dominance and persistence of a particular epidemiological clon…

2012

In this study, using multilocus microsatellite analysis, we report the genetic characterization of 27 Candida parapsilosis isolates recovered in two different periods of time (2007-2009 and 2011-2012) from infants hospitalized in the neonatal intensive care unit of a hospital in Messina, Italy. The results revealed the persistence and dominance of a particular infectious genotype among NICU patients and highlight the power of the used microsatellite markers in clarifying epidemiologic associations, detect micro-evolutionary variations and facilitating the recognition of outbreaks. © 2012 Elsevier B.V.

Microbiology (medical)medicine.medical_specialtyCandida parapsilosisNeonatal intensive care unitGenotypeSettore MED/17 - Malattie InfettiveInfectious DiseaseCandida parapsilosisMicrobiologyDisease OutbreaksCandida orthopsilosisGeneticCandida orthopsilosiIntensive Care Units NeonatalEpidemiologyGenotypeGeneticsmedicineHumansMolecular BiologyGenotypingEcology Evolution Behavior and SystematicsCandidaDominance (genetics)ITS-sequencingGeneticsCross InfectionDisease OutbreakbiologyCandidiasisInfant NewbornOutbreakCandida metapsilosisbiology.organism_classificationCandida parapsilosis; Candida orthopsilosis; Candida metapsilosis; Molecular epidemiology; ITS-sequencing; Microsatellite genotypingEcology Evolution Behavior and SystematicCandida metapsilosiInfectious DiseasesMolecular epidemiologyCandidiasiCandida parapsilosiMicrosatellite RepeatMicrosatelliteMicrosatellite genotypingMicrosatellite RepeatsHumanMultilocus Sequence TypingInfection, Genetics and Evolution
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Yeast Killer Toxin-Like Candidacidal Ab6 Antibodies Elicited through the Manipulation of the Idiotypic Cascade

2014

A mouse anti-anti-anti-idiotypic (Id) IgM monoclonal antibody (mAb K20, Ab4), functionally mimicking a Wyckerhamomyces anomalus (Pichia anomala) killer toxin (KT) characterized by fungicidal activity against yeasts presenting specific cell wall receptors (KTR) mainly constituted by β-1,3-glucan, was produced from animals presenting anti-KT Abs (Ab3) following immunization with a rat IgM anti-Id KT-like mAb (mAb K10, Ab2). MAb K10 was produced by immunization with a KT-neutralizing mAb (mAb KT4, Ab1) bearing the internal image of KTR. MAb K20, likewise mAb K10, proved to be fungicidal in vitro against KT-sensitive Candida albicans cells, an activity neutralized by mAb KT4, and was capable of…

beta-GlucansPhage displayImmunogenPichia anomalaHumoral Immune ResponseAntibody Responselcsh:MedicinePichiaMiceCandida albicansVaccines DNAlcsh:ScienceImmune ResponseMultidisciplinaryVaccinationCandidiasisInfectious Disease ImmunologyKiller Factors YeastAntibodies Anti-IdiotypicVaccines SubunitResearch Articlemedicine.drug_classMolecular Sequence DataImmunologyReceptors Cell SurfaceMycologyBiologyMonoclonal antibodyMicrobiologyMicrobiologyFungal ProteinsAntigenPeptide LibrarymedicineAnimalsAmino Acid SequencePeptide libraryFungal vaccineMolecular Mimicrylcsh:RImmunityBiology and Life Sciencestossina killer mAb K20 Anti-idiotypic peptide mimic candidacidal activityMycotoxinsMolecular biologyRatsHemocyaninsHumoral Immunitybiology.proteinClinical Immunologylcsh:QFungal VaccinesPeptidesKeyhole limpet hemocyaninPLoS ONE
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Potential association of specific Candida parapsilosis genotypes, bloodstream infections and colonization of health workers' hands.

2014

AbstractFungal nosocomial infections continue to be a serious problem among hospitalized patients, decreasing quality of life and adding millions of euros to healthcare costs. The aim of this study was to describe the pattern of fungi associated with the hands of healthcare workers and to genotype Candida parapsilosis isolates in order to understand whether their high clinical prevalence stems from endemic nosocomial genotypes or from the real emergence of epidemiologically-unrelated strains. Approximately 39% (50/129) of healthcare workers were positive for yeasts and among 77 different fungal isolates recovered, C. parapsilosis was the most frequent (44/77; 57%). Twenty-seven diverse geno…

Microbiology (medical)Candida parapsilosismicrosatellite genotypingSettore MED/17 - Malattie InfettiveGenotypeSepsiHospitalized patientsHealth PersonnelFungal pathogenNosocomial fungal infectionInfectious DiseaseBiologyCandida parapsilosisPersistence (computer science)MicrobiologyCandida parapsilosis; Candida species; Candidaemia; fungal pathogens; healthcare workers; microsatellite genotyping; nosocomial fungal infectionsCandidaemiaRetrospective Studienosocomial fungal infectionsSepsisGenotypeCandida speciesDisease Transmission InfectiousHumansColonizationDNA FungalMycological Typing TechniquesCandidaRetrospective StudiesGenetic diversityCross InfectionMolecular Epidemiologyhealthcare workersMedicine (all)Candida parapsilosis; Candida species; Candidaemia; Fungal pathogens; Healthcare workers; Microsatellite genotyping; Nosocomial fungal infections; Candida; Cross Infection; DNA Fungal; Disease Transmission Infectious; Genotype; Hand; Humans; Molecular Epidemiology; Molecular Typing; Mycological Typing Techniques; Retrospective Studies; Sepsis; Health Personnel; Microbiology (medical); Infectious Diseases; Medicine (all)General Medicinebiology.organism_classificationHandMolecular TypingInfectious DiseasesDisease Transmission InfectiouMicrosatellite AnalysisCandida specieHealthcare workerCandida parapsilosiMycological Typing Techniquefungal pathogensHumanClinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
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