6533b821fe1ef96bd127ae90

RESEARCH PRODUCT

Microsatellite-based genotyping of Candida parapsilosis sensu stricto isolates reveals dominance and persistence of a particular epidemiological clone among neonatal intensive care unit patients

Daniela Petronilla RomeoOrazio RomeoCarla Lo PassoDemetrio DelfinoI PerniceMaria AmoriniAntonio Cascio

subject

Microbiology (medical)medicine.medical_specialtyCandida parapsilosisNeonatal intensive care unitGenotypeSettore MED/17 - Malattie InfettiveInfectious DiseaseCandida parapsilosisMicrobiologyDisease OutbreaksCandida orthopsilosisGeneticCandida orthopsilosiIntensive Care Units NeonatalEpidemiologyGenotypeGeneticsmedicineHumansMolecular BiologyGenotypingEcology Evolution Behavior and SystematicsCandidaDominance (genetics)ITS-sequencingGeneticsCross InfectionDisease OutbreakbiologyCandidiasisInfant NewbornOutbreakCandida metapsilosisbiology.organism_classificationCandida parapsilosis; Candida orthopsilosis; Candida metapsilosis; Molecular epidemiology; ITS-sequencing; Microsatellite genotypingEcology Evolution Behavior and SystematicCandida metapsilosiInfectious DiseasesMolecular epidemiologyCandidiasiCandida parapsilosiMicrosatellite RepeatMicrosatelliteMicrosatellite genotypingMicrosatellite RepeatsHumanMultilocus Sequence Typing

description

In this study, using multilocus microsatellite analysis, we report the genetic characterization of 27 Candida parapsilosis isolates recovered in two different periods of time (2007-2009 and 2011-2012) from infants hospitalized in the neonatal intensive care unit of a hospital in Messina, Italy. The results revealed the persistence and dominance of a particular infectious genotype among NICU patients and highlight the power of the used microsatellite markers in clarifying epidemiologic associations, detect micro-evolutionary variations and facilitating the recognition of outbreaks. © 2012 Elsevier B.V.

yearjournalcountryeditionlanguage
2012-07-24Infection, Genetics and Evolution
https://doi.org/10.1016/j.meegid.2012.09.006