0000000000498080

AUTHOR

Tommaso Nastasi

showing 2 related works from this author

RNA-binding ability of PIPP in requires the entire protein

2003

Post-transcriptional fate of eukaryotic mRNAs depends on association with different classes of RNA-binding proteins (RBPs). Among these proteins, the cold-shock domain (CSD)-containing proteins, also called Y-box proteins, play a key role in controlling the recruitment of mRNA to the translational machinery, in response to environmental cues, both in development and in differentiated cells. We recently cloned a rat cDNA encoding a new CSD-protein that we called PIPPin. This protein also contains two putative double-stranded RNA-binding motifs (PIP(1) and PIP(2)) flanking the central CSD, and is able to bind mRNAs encoding H1 degrees and H3.3 histone variants. In order to clarify the role of…

Protein FoldingNerve Tissue ProteinsSequence alignmentRNA-binding proteinPlasma protein bindingArticleRNA-binding proteinscold-shock domainPIPPinhistone variantsHistonesSettore BIO/10 - BiochimicaComplementary DNAHistone H2AAnimalsRNA MessengerGeneticsMessenger RNAbiologyRNA-Binding ProteinsRNACell BiologyRecombinant ProteinsProtein Structure TertiaryRatsCell biologyHistoneGene Expression Regulationbiology.proteinMolecular MedicineSequence AlignmentProtein BindingJournal of Cellular and Molecular Medicine
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Expression of synapsin I gene in primary cultures of differentiating rat cortical neurons

1995

Synapsin I is a neuron-specific protein which is present in two isoforms, Ia and Ib. In the last few years this protein has been demonstrated to play a central role in the regulation of neurotransmitter release and synaptic plasticity. In this paper the developmental expression of this protein has been investigated in primary neuronal cultures from fetal rat brain cortices. The presence of thyroid hormone in the culture medium stimulates an early expression of the protein without exerting any effect at the level of mRNA transcription and accumulation. These observations implicate a T3-dependent regulation of this neuron-specific gene at the level of mRNA translation. © 1995 Plenum Publishin…

Gene isoformmedicine.medical_specialtySynapsin ITime FactorsTranscription GeneticBlotting Westernsynapsin IGene ExpressionBiologyBiochemistryCellular and Molecular Neurosciencechemistry.chemical_compoundFetusInternal medicineSettore BIO/10 - BiochimicaGene expressionmedicineAnimalsRNA MessengerNeurotransmitterCells CulturedCell NucleusCerebral CortexNeuronsMessenger RNANeuroscience (all)Cell DifferentiationGeneral MedicineSynapsinBlotting NorthernSynapsinsthyroid hormoneRatsCell biologyKineticsmedicine.anatomical_structureEndocrinologynervous systemchemistryNeuronal differentiationSynaptic plasticityTriiodothyronineSettore MED/26 - NeurologiaNeuron
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