0000000000505391
AUTHOR
Monika Neuhäuser
Determination of N-acetyl-l-glutamine in urine by HPLC
The determination of N-acetyl-l-glutamine (AC-GLN) in urine of rats after derivatization with xanthydrol by HPLC is described. The urine samples were collected from two groups of rats, one group fed normal nutrition, the other treated with AC-GLN. About 5 mM/l AC-GLN was detected in urine of untreated animals, whereas the excretion of the other group was in the range of 50 mM/l corresponding to about 50 wt.-% of the given AC-GLN.
Endogenous 3-methylhistidine excretion in healthy women and men with reference to muscle protein metabolism.
Presently 3-methylhistidine excretion is widely used for monitoring the metabolic status of patients during different kinds of clinical conditions. Aim of the study was to reconsider its predicative value on the basis of a larger collective of healthy persons and to find a standardization independent from sex. Therefore endogenous 3-methylhistidine release of 40 healthy adults (24 women and 16 men) was measured and related to body weight, body surface area, arm muscle circumference, and nitrogen and creatinine excretion. A positive correlation could be observed only for 3-methylhistidine and creatinine excretion and that to the same extent both for females and males. Assuming that the excre…
Zur Frage der Verwertung von N-acetylierten Aminosäuren in Infusionslösungen. Die Spaltung von N-Acetyltyrosin und N-Acetylcystein in Rattengeweben
N-Acetyl-L-tyrosin und N-Acetyl-L-cystein werden in vitro durch Enzympraparate aus Niere und Leber der Ratte gespalten. Wahrscheinlich handelt es sich um zwei verschiedene Acylasen. Michaelis-Konstanten und Aktivitaten werden angegeben. Niere enthalt pro Gewichtseinheit wesentlich mehr Aktivitat als Leber. In der Muskulatur wird keine mesbare Aktivitat gefunden.