0000000000515149

AUTHOR

Amy Lynn Aulthouse

showing 3 related works from this author

In-vitro Proteoglykansynthese in redifferenzierten Chondrozyten

1989

Human chondrocytes growing in monolayer cultures de-differentiate and produce type I collagen. They re-differentiate and resume their in-vivo characteristics (including the production of type II collagen) when cultured in an agarose-gel. To characterize the modulated cells in more detail, biochemical studies were performed in chondrocytes suspended in agarose for 1 to 3 weeks.

chemistry.chemical_compoundchemistryMonolayerType II collagenAgaroseMolecular biologyIn vitroType I collagen
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Proteoglycan synthesis by cultured human chondrocytes.

1994

Iliac crest biopsies are important in the detection of human skeletal dysplasias. Therefore, culture of these cells may serve as a valuable method for studying proteoglycan metabolism in chondrocytes of individuals with skeletal abnormalities. Morphological and biochemical studies were performed on human iliac crest chondrocytes grown in monolayer and in agarose gels. Two proteoglycan populations of different hydrodynamic size and glycosaminoglycan composition were synthesized by cells grown in monolayer. Chondrocytes cultured in an agarose gel for 2 weeks synthesized proteoglycans identical to those of the native tissue with respect to hydrodynamic size and glycosaminoglycan chain length. …

HistologyAscorbic AcidChondrocyteGlycosaminoglycanIliumchemistry.chemical_compoundmedicineHumansInstrumentationCells CulturedGlycosaminoglycansbiologyChemistryCartilageSepharoseChondroitin SulfatesInfant NewbornCell DifferentiationAscorbic acidCell biologycarbohydrates (lipids)Medical Laboratory Technologymedicine.anatomical_structureCartilageBiochemistryProteoglycanChondroitin Sulfate ProteoglycansCell culturebiology.proteinUltrastructureChromatography GelAgaroseAnatomyMicroscopy research and technique
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Influence of retinol on human chondrocytes in agarose culture

1992

Vitamin A and its congeners, collectively called retinoids, are known to have teratogenic potential and have induced craniofacial and limb malformations in numerous animal species. More importantly, retinoids are recognized as teratogenic to fetuses of pregnant women who have taken such preparations for dermatologic disorders. Information gathered from the study of animal models suggests that retinoids interfere with cartilage differentiation. If chondrogenesis in limb development is disturbed it may contribute to limb reductions and malformations. In vitro studies using various animal systems have shown that cartilage matrix macromolecules are altered to resemble those secreted by mesenchy…

SepharoseCartilageMesenchymal stem cellType II collagenBiologyChondrogenesisAgricultural and Biological Sciences (miscellaneous)ChondrocyteCartilageTeratogensmedicine.anatomical_structureBiochemistryProteoglycanCell culturemedicinebiology.proteinHumansProteoglycansCollagenAnatomyVitamin ACells CulturedType I collagenThe Anatomical Record
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