0000000000522506
AUTHOR
Rocío Daniela Inés Molina
Inhibition of bacterial virulence factors of foodborne pathogens by paprika (Capsicum annuum L.) extracts
Abstract Capsicum annuum extracts of different polarities were tested for their antimicrobial, antibiofilm, and anti-Quorum sensing (QS) activity against several pathogenic microorganisms and possible toxic effects using an in vivo model (Galleria mellonella L.). Hexane (HE) and Chloroform extract (CE) were the most active in inhibiting biofilm formation of the eight bacteria tested, with inhibition percentages between 22 and 88% for a concentration of 100 μg/mL. These extracts were potent inhibitors of Staphylococcus aureus biofilm, and microscopy analysis confirmed a significant reduction in adhered cells. Moreover, polystyrene surfaces coated with these extracts decreased Staph. aureus a…
Antibiofilm activity of coriander (Coriander sativum L.) grown in Argentina against food contaminants and human pathogenic bacteria
Abstract Bacterial spoilage of food and other infections are a coordinated mechanism controlled by quorum sensing (QS), which regulate the expression of virulence factors, such as biofilm. The antimicrobial, antibiofilm, anti-QS and anti-swarming properties of hexane (HE), chloroform (CE), ethyl acetate (EAE), methanolic (ME) and total methanolic (TME) extracts of coriander grown in Argentina were investigated. In vivo extracts toxicity using Galleria mellonella L. model and chemical composition (GC-MS) were also assessed. The antibiofilm activity of polystyrene surfaces coated with the most active extracts was evaluated. At non-inhibitory growth concentrations, extracts showed a strong ant…
Laurel extracts inhibit quorum sensing, virulence factors and biofilm of foodborne pathogens
Antimicrobial, antibiofilm, anti-Quorum sensing (QS) and virulence factors inhibitory capacity of different polarity Laurus nobilis extracts against several pathogenic microorganisms were studied. Some extracts exhibited antibiotic effect against Staphylococcus aureus multidrug-resistant strains. However, all extracts (100 µg/mL) inhibited to some extent the biofilm of most bacteria tested (until 40% for Gram-negative and 76% for Gram-positive). Hexane (HE) and chloroform extract (CE) were potent inhibitors of S. aureus biofilm and the microscopies further confirmed an important reduction in adherent cells. Polystyrene surfaces coated with these extracts showed a decrease in bacterial adhes…