6533b82bfe1ef96bd128e1a8

RESEARCH PRODUCT

Laurel extracts inhibit quorum sensing, virulence factors and biofilm of foodborne pathogens

Mario Eduardo ArenaAlexandre José MacedoMyriam Anabel DíazMaría Amparo BlázquezRodrigo Campos-silvaMaría Rosa AlbertoRocío Daniela Inés Molina

subject

0106 biological sciencesPYOCYANINVirulenceSwarming motilitymedicine.disease_cause01 natural sciencesMicrobiology//purl.org/becyt/ford/1 [https]chemistry.chemical_compound0404 agricultural biotechnologyPyocyanin010608 biotechnologymedicine//purl.org/becyt/ford/1.6 [https]biologyENZYME INHIBITIONPseudomonas aeruginosaChemistryBiofilm04 agricultural and veterinary sciencesbiology.organism_classification040401 food scienceSWARMINGQuorum sensingStaphylococcus aureusBIOFILMBacteriaFATTY ACIDSFood Science

description

Antimicrobial, antibiofilm, anti-Quorum sensing (QS) and virulence factors inhibitory capacity of different polarity Laurus nobilis extracts against several pathogenic microorganisms were studied. Some extracts exhibited antibiotic effect against Staphylococcus aureus multidrug-resistant strains. However, all extracts (100 µg/mL) inhibited to some extent the biofilm of most bacteria tested (until 40% for Gram-negative and 76% for Gram-positive). Hexane (HE) and chloroform extract (CE) were potent inhibitors of S. aureus biofilm and the microscopies further confirmed an important reduction in adherent cells. Polystyrene surfaces coated with these extracts showed a decrease in bacterial adhesion with the resulting inhibition of S. aureus biofilm formation and biofilm activity. Moreover, they also interfere with the coagulase and hemolysin activities of this bacterium. With respect to Gram-negative bacteria, these extracts showed anti-QS activity against Chromobacterium violaceum and inhibited biofilm formation, swarming motility, pyocyanin production, and elastase activity of Pseudomonas aeruginosa. Chemical analysis of HE and CE revealed that the fatty acids were the main compounds, particularly linolenic, oleic and hexadecanoic acids followed by the oxygenated monoterpenes. Non-toxic laurel extracts offer a natural alternative to control contamination and/or spoilage of food, as well as infectious diseases, by attacking the biofilm and the virulence of Gram-positive and Gram-negative bacteria. Fil: Molina, Rocío Daniela Inés. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina Fil: Campos Silva, Rodrigo. Universidade Federal do Rio Grande do Sul; Brasil Fil: Díaz, Myriam Anabel. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina Fil: Macedo, Alexandre José. Universidade Federal do Rio Grande do Sul; Brasil Fil: Blázquez, María Amparo. Universidad de Valencia; España Fil: Alberto, Maria Rosa. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Arena, Mario Eduardo. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina

yearjournalcountryeditionlanguage
2020-12-01
10.1016/j.lwt.2020.109899https://www.sciencedirect.com/science/article/pii/S0023643820308884