0000000000546253
AUTHOR
E. Navarro
Microbial Diversity in the Midguts of Field and Lab-Reared Populations of the European Corn Borer Ostrinia nubilalis
Background: Insects are associated with microorganisms that contribute to the digestion and processing of nutrients. The European Corn Borer (ECB) is a moth present world-wide, causing severe economical damage as a pest on corn and other crops. In the present work, we give a detailed view of the complexity of the microorganisms forming the ECB midgut microbiota with the objective of comparing the biodiversity of the midgut-associated microbiota and explore their potential as a source of genes and enzymes with biotechnological applications. Methodological/Principal Findings: A high-throughput sequencing approach has been used to identify bacterial species, genes and metabolic pathways, parti…
Monte Carlo simulation of alpha spectra in low-geometry measurements
Abstract Monte Carlo simulation of alpha spectra obtained with semiconductor detectors in low-geometry has been developed. The proposed method reproduces adequately experimental spectra of alpha emitters by taking into account the energy losses and trajectory changes of alpha particles as consequences of their interactions in the source, backing and detector.
Solution NMR structure of aD,L-alternating oligonorleucine as a model of ?-helix
beta-Helix structures are of particular interest due to their capacity to form transmembrane channels with different transport properties. However, the relatively large number of beta-helices configurations does not allow a direct conformational analysis of beta-helical oligopeptides. A synthetic alternating D,L-oligopeptide with twelve norleucines (XIIMe) has been used as a model to get insight in the conformational features of beta-helix structures. The spatial configuration of XIIMe in solution has been determined by NMR. An extensive set of distances (nuclear Overhauser effect) and dihedral (J coupling constants) constraints have been included in molecular dynamics calculations. The NMR…
Radon levels in the Valencian community (Spain). I. Results in the province of Valencia (1990–1991)
Abstract Two-day radon screening measurements with charcoal canisters were made in buildings in the province of Valencia throughout the years 1990 and 1991. An average indoor concentration of 34 Bq m −3 was found, and 9% of all measurements exceeded the recommended EPA limit of 148 Bq m −3 .
La osteotomia en la coxartrosis (30 casos)
30 cases of osteoarthritis of the hip treated with varus femoral osteotomy are colIected. The surgical technique and results are described. The latter postop X-rays suggest a remodelling and growth of the articular cartilage of the head and also of the acetabulum.
Estudio comparativo de las prótesis no cementadas de cerámica tipo mittelmeier utilizando el sistema adhesivo de fibrina
Fourty cases of Total Hip replacement with the MITTELMEIER's prothesis are reported. In 20 of them a fibrine coating was used in order to improve the biological setting of the acetabulum and femoral components. Ten cases were operated following the original Mittelmeier's technique. The results in both series are very similar, but the authors suggest the valuable use of the fibrine to estimulate the ossification around the prothesis component for a quicker biological fixation.
Lorentzian Comments on Stokes Parameters
The popular Stokes statements about polarized light are interpreted in a Minkowskian language using a Lorentzian representation for the Stokes parameters and the degree of polarization. The evolution equations for Stokes parameters on a curved space-time are obtained using the parallel transport of the polarization vector along a null geodesic. The interest of these equations in Astrophysics and Relativistic Cosmology is outlined.
Intercomparison of soil moisture retrieved from GNSS-R and passive L-band radiometry at the Valencia Anchor Station
In this paper, the SOMOSTA (Soil Moisture Monitoring Station) experiment on soil moisture monitoring by Global Navigation Satellite System Reflected signals(GNSS-R) at the Valencia Anchor Station is introduced.
Reconstruction and analysis of genome-scale metabolic model of a photosynthetic bacterium
Abstract Background Synechocystis sp. PCC6803 is a cyanobacterium considered as a candidate photo-biological production platform - an attractive cell factory capable of using CO2 and light as carbon and energy source, respectively. In order to enable efficient use of metabolic potential of Synechocystis sp. PCC6803, it is of importance to develop tools for uncovering stoichiometric and regulatory principles in the Synechocystis metabolic network. Results We report the most comprehensive metabolic model of Synechocystis sp. PCC6803 available, iSyn669, which includes 882 reactions, associated with 669 genes, and 790 metabolites. The model includes a detailed biomass equation which encompasses…
Resultado a corto plazo de la artroplastia no cementada modelo Mittelmeier
Los autores han revisado 56 Artroplastias de Cadera no cementada empleando prótesis de Cerámica. Describen su técnica quirúrgica exponiendo los resultados obtenidos. Todos estos pacientes han sido operados en el Hospital Insular de las Palmas de Gran Canaria, en el periodo comprendido entre 1984 y 1988. 56 Cementedless Total Hip Prothesis of Ceramic are colected. The authors describe their surgical procedure showing the results obtained.
An electrical current smart transducer based on PSoC platform and integrated spin-valve sensor with embedded thin film Ruthenium temperature sensor
This work presents an intelligent electrical current sensor that combines a programmable system on chip (PSoC) with a microfabrication of a giant magnetoresistance sensor (GMR) and resistive temperature detector (RTD). Four spin-valve GMR elements configured in a Wheatstone bridge sense the electrical current, while a thin film RTD based on Ruthenium measures the temperature for spin-valve bridge sensitivity thermal compensation. The mixed signal PSoC allows implement in a single chip all electronic circuits needed to design a smart transducer: the spin-valve biasing, conditioning signal, analog to digital converter, digital signal processing and ports for communications. In the experimenta…
Vanillin cell sensor
Our project for iGEM 2006 consisted of designing a cellular vanillin biosensor. We used an EnvZ -E. coli strain as a chassis, and constructed two different devices: a sensor and an actuator, assembled using OmpR-P as a standardised mediator. The sensor device contained a computation- ally designed vanillin receptor and a synthetic two-component signal transduction protein (Trz). The receptor protein was based on a ribose-binding protein as scaffold. The Trz was built by fusion of the periplasmic and transmembrane domains of a Trg protein with an EnvZ kinase domain. When the receptor complex binds Trg, an allosteric motion is propagated to the cyto- plasmic EnvZ kinase domain, resulting in a…
GEOLOGIA DE LAS MARGENES DE LA PLACA DEL CARIBE: GENERALIDADES EN GUATEMALA, COSTA RICA, LA ESPAÑOLA Y RESULTADOS PRELIMINARES DEL ANALISIS DE UNA TRANSVERSAL EN LA CORDILLERA DE LA COSTA DE VENEZUELA
The Caribbean Plate margins are constituted by deformed belts built up since the Cretaceous in acompressional and strike-slip stress field, which allowed overthrusting of the Caribbean crust onto the Pacific,North and South American Plates.The Caribbean borders include Jurassic-Cretaceous ophiolitic units (Great Antilles, Venezuela, Costa Rica,Guatemala, etc.), composed by mantle peridotites, gabbros, volcanic and sedimentary covers, which have beendeformed in at least two ductile penetrative phases and were often metamorphosed in the prehnite-pumpelleyite,green and blue schist, amphibolite, and in places eclogite facies. These units may present part of a subductioncomplex or are an accreti…
Minkowskian description of polarized light and polarizers.
A conventional Stokes description of polarized light is considered in a four-dimensional Lorentzian space, developing a seminal idea of Paul Soleillet [Ann. Phys. (Paris) 12, 23 (1929)]. This provides a striking interpretation for the degree of polarization and the Stokes decomposition of light beams. Malus law and reciprocity theorems for polarizers are studied using this Lorentzian formalism.
Infecciones nosocomiales versus Staphylococcus: estudio comparativo con y sin utilización de osteosíntesis
Se ha estudiado durante el periodo comprendido entre el 31 de Diciembre de 1987 hasta el 1 de Enero de 1989, las infecciones nosocomiales detectadas en el Servicio de Traumatología y Cirugía Ortopédica en los 11O1 enfermos ingresados en este periodo y en los 1383 tratamientos realizados sobre los mismos, atendiendo a riguroso criterio de infección, tanto clínico (leucocitosis, fiebre, desviación a la izquierda del esquema de Amold y supuración) como microbiológico aislamiento e identificación del gérmen causal). Se han detectado 44 infecciones, de las cuales, 30 han sido por Staphylococus y el resto por enterobacterias, de las cuales, 4 han sido infecciones urinarias por sondaje vesical. A …
Aequorin-expressing yeast emits light under electric control
In this study, we show the use of direct external electrical stimulation of a jellyfish luminescent calcium-activated protein, aequorin, expressed in a transgenic yeast strain. Yeast cultures were electrically stimulated through two electrodes coupled to a standard power generator. Even low (1.5. V) electric pulses triggered a rapid light peak and serial light pulses were obtained after electric pulses were applied periodically, suggesting that the system is re-enacted after a short refraction time. These results open up a new scenario, in the very interphase between synthetic biology and cybernetics, in which complex cellular behavior might be subjected to electrical control.
Conformational and structural analysis of the equilibrium between single- and double-strand ?-helix of aD,L-alternating oligonorleucine
Alternating sequences of D and L residues in peptides are directly related to the formation of several kinds of regular helical conformations usually called beta-helices. The major feature of these structures is that they can be associated with the transmembrane ion-conducting channel activity in some natural antibacterial peptides. The study of alternating D,L synthetic peptides is critical to understand how factors such as surrounding media, main chain length, type of side chain and terminal groups, among others, can determine the adoption of a specific kind of beta-helix. Early studies pointed out that the peptides Boc-(D-NLeu-L-NLeu)(6)-D-MeNLe-L-Nl-D-Nl-L-Nl-OMe (Boc: tert-butyloxycarb…
Natural and artificial radioactivity levels in Livingston Island (Antarctic regions).
Radioactive contamination of the sea and land is due, on the one hand, to fallout from atmospheric atomic explosions since 1945, and, on the other, to emissions produced by nuclear and radioactive facilities. Given its geographic position far distant from the aforementioned main sources of radioactive contamination, Antarctica should have the lowest levels that can be measured on the Earth of artificial radionuclides in the various receptor media which are characteristic of the trophic chain. In the case of Antarctica, these are melt-water, sea-water, mosses, algae, and lichens. With the aim of contributing basic information on the radiation levels present in the Antarctic ecosystem, we hav…
On complete set of solutions for polynomial matrix equations
Abstract In this paper we introduce the concept of co-solution of a polynomial matrix equation which permits us to obtain necessary and sufficient conditions so that a set of solutions be a complete set.
Short and medium effects on the environment of Valencia, Spain, of the Chernobyl nuclear plant accident.
As a consequence of the 26 April 1986 accident at the Chernobyl nuclear plant, a large amount of radioactivity was released into the atmosphere. The radioactive plume formed could be detected in practically the whole of the Northern Hemisphere a few days later. The zone most affected by the radioactive cloud over Spain was that of the Mediterranean coast and the Balearic Islands. In this paper, the authors examine the level of the radioactive contamination reached in various receptive media in Valencia, such as air, dry-fallout, water, soil, grass and milk samples collected in Valencia immediately after the accident. The activity levels are compared with those found during 1964 and 1965 due…
Solution structure of aD,L-alternating oligonorleucine as a model of double-stranded antiparallel ?-helix
Conformational characteristics of alternating D,L linear peptides are of particular interest because of their capacity to form transmembrane channels with different transport properties, as some natural antibiotics do. Single- and double-stranded beta-helical structures are common for alternating D,L peptides. The stability of the beta-helix depends on several structural factors, such as the backbone peptide length, type and position of side chains, and nature of terminal groups. The NMR and molecular dynamics solution conformation of a synthetic alternating D,L-oligopeptide with 15 norleucines (XVMe) has been used as a model to get insight in to the conformational features of double-strand…
Yeast cultures with UCP1 uncoupling activity as a heating device
7 páginas, 5 figuras, 3 tablas -- PAGS nros. 300-306