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RESEARCH PRODUCT
Aequorin-expressing yeast emits light under electric control
ÁNgeles HuesoÁNgeles HuesoGuillem MarcoGuillem MarcoJoaquin ArinoLaura Domínguez-escribáArnau MontagudCristina VilanovaE. NavarroJuny CrespoJuny CrespoJavier F. UrchueguíaCarles PalancaCarles PalancaAsier GonzálezManuel PorcarAndrés MoyaEduardo OteroEduardo OteroPedro Fernández De CórdobaM. PitarchM. PitarchJerzy O. SzablowskiJerzy O. SzablowskiSara RiveraSara Riverasubject
Activated proteinsLightScyphozoaAequorinApplied Microbiology and BiotechnologyElectricityHydroxidesCellular behaviorsPriority journalYeast electro-stimulationbiologyPhosphorescencePower generatorsGeneral MedicineElectrostimulationMAQUINAS Y MOTORES TERMICOSElectrodeElectric pulseInterphaseBioluminescenceYeast cultureGenetic EngineeringMATEMATICA APLICADABiotechnologyPotassium CompoundsLight pulse generatorsSaccharomyces cerevisiaeBioengineeringNanotechnologyLight pulseSaccharomyces cerevisiaeElectrical stimulationsYeast strainArticleAequorinBioluminescenceBiologySynthetic biologyBioelectronicsBioelectronicsbiology.organism_classificationElectric controlYeastElectric StimulationYeastFISICA APLICADABiophysicsbiology.proteinProtein expressionJellyfishElectrical controlLuminescenceCell functionTransgenicsdescription
In this study, we show the use of direct external electrical stimulation of a jellyfish luminescent calcium-activated protein, aequorin, expressed in a transgenic yeast strain. Yeast cultures were electrically stimulated through two electrodes coupled to a standard power generator. Even low (1.5. V) electric pulses triggered a rapid light peak and serial light pulses were obtained after electric pulses were applied periodically, suggesting that the system is re-enacted after a short refraction time. These results open up a new scenario, in the very interphase between synthetic biology and cybernetics, in which complex cellular behavior might be subjected to electrical control.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2011-01-01 |