0000000000557581

AUTHOR

Isabelle Caillé

showing 4 related works from this author

Multipotent Neural Stem Cells Reside into the Rostral Extension and Olfactory Bulb of Adult Rodents

2002

The lateral walls of the forebrain lateral ventricles are the richest source of stem cells in the adult mammalian brain. These stem cells give rise to new olfactory neurons that are renewed throughout life. The neurons originate in the subventricular zone (SVZ), migrate within the rostral extension (RE) of the SVZ along the rostral migratory stream (RMS) within tube-like structures formed of glial cells, to eventually reach the olfactory bulb (OB). We demonstrate that, contrary to the current view, multipotential (neuronal-astroglial-oligodendroglial) precursors with stem cell features can be isolated not only from the SVZ but also from the entire RE, including the distal portion within the…

Time FactorsRostral migratory streamanimal diseasesCell Culture TechniquesSubventricular zoneCell SeparationBiologyCell LineMiceCell MovementLateral VentriclesSpheroids CellularNeurospheremedicineAnimalsARTICLEGrowth SubstancesCells CulturedNeuronsNeurotransmitter AgentsStem CellsGeneral NeuroscienceNeurogenesisCell DifferentiationOlfactory BulbNeural stem cellClone CellsNeuroepithelial cellOligodendrogliaPhenotypemedicine.anatomical_structurenervous systemAstrocytesStem cellNeuroscienceCell DivisionAdult stem cellThe Journal of Neuroscience
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EGF converts transit-amplifying neurogenic precursors in the adult brain into multipotent stem cells.

2002

AbstractNeural stem cells in the subventricular zone (SVZ) continue to generate new neurons in the adult brain. SVZ cells exposed to EGF in culture grow to form neurospheres that are multipotent and self-renewing. We show here that the majority of these EGF-responsive cells are not derived from relatively quiescent stem cells in vivo, but from the highly mitotic, Dlx2+, transit-amplifying C cells. When exposed to EGF, C cells downregulate Dlx2, arrest neuronal production, and become highly proliferative and invasive. Killing Dlx2+ cells dramatically reduces the in vivo response to EGF and neurosphere formation in vitro. Furthermore, purified C cells are 53-fold enriched for neurosphere gene…

Cellular differentiationNeuroscience(all)Mice TransgenicBiology03 medical and health sciencesMice0302 clinical medicineCell MovementNeurosphereSpheroids CellularAnimalsCell LineageCells Cultured030304 developmental biologyHomeodomain ProteinsNeurons0303 health sciencesEpidermal Growth FactorGeneral NeuroscienceStem CellsBrainCell DifferentiationImmunohistochemistryNeural stem cellCell biologyUp-RegulationNeuroepithelial cellEndothelial stem cellErbB ReceptorsMicroscopy ElectronPhenotypenervous systemMultipotent Stem CellAstrocytesStem cellNeuroscience030217 neurology & neurosurgeryCell DivisionAdult stem cellTranscription FactorsNeuron
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Subventricular Zone Astrocytes Are Neural Stem Cells in the Adult Mammalian Brain

1999

AbstractNeural stem cells reside in the subventricular zone (SVZ) of the adult mammalian brain. This germinal region, which continually generates new neurons destined for the olfactory bulb, is composed of four cell types: migrating neuroblasts, immature precursors, astrocytes, and ependymal cells. Here we show that SVZ astrocytes, and not ependymal cells, remain labeled with proliferation markers after long survivals in adult mice. After elimination of immature precursors and neuroblasts by an antimitotic treatment, SVZ astrocytes divide to generate immature precursors and neuroblasts. Furthermore, in untreated mice, SVZ astrocytes specifically infected with a retrovirus give rise to new n…

MaleRostral migratory streamanimal diseasesSubventricular zoneChick EmbryoBiologyGeneral Biochemistry Genetics and Molecular BiologyCerebral VentriclesSubgranular zoneMice03 medical and health sciences0302 clinical medicineNeuroblastNeurosphereGlial Fibrillary Acidic ProteinmedicineAnimalsRegeneration030304 developmental biology0303 health sciencesBiochemistry Genetics and Molecular Biology(all)Stem CellsBrainAnatomyOlfactory BulbNeural stem cell3. Good healthCell biologyNeuroepithelial cellmedicine.anatomical_structureNeuropoiesisnervous systemAstrocytes030217 neurology & neurosurgeryCell
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Lack of the Cell-Cycle Inhibitor p27Kip1 Results in Selective Increase of Transit-Amplifying Cells for Adult Neurogenesis

2002

The subventricular zone (SVZ) is the largest germinal layer in the adult mammalian brain and comprises stem cells, transit-amplifying progenitors, and committed neuroblasts. Although the SVZ contains the highest concentration of dividing cells in the adult brain, the intracellular mechanisms controlling their proliferation have not been elucidated. We show here that loss of the cyclin-dependent kinase inhibitor p27Kip1 has very specific effects on a population of CNS progenitors responsible for adult neurogenesis. Using bromodeoxyuridine and [3H]thymidine incorporation to label cells in S phase and cell-specific markers and electron microscopy to identify distinct cell types, we compared th…

MaleCell typePopulationSubventricular zoneApoptosisCell CountCell Cycle ProteinsMice TransgenicBiologyMicechemistry.chemical_compoundNeuroblastLateral VentriclesSpheroids CellularIn Situ Nick-End LabelingmedicineAnimalsARTICLEProgenitor celleducationCells CulturedNeuronseducation.field_of_studyStem CellsTumor Suppressor ProteinsGeneral NeuroscienceCell CycleNeurogenesisCell DifferentiationImmunohistochemistryCell biologyMice Inbred C57BLmedicine.anatomical_structureBromodeoxyuridinenervous systemchemistryStem cellCell DivisionCyclin-Dependent Kinase Inhibitor p27BromodeoxyuridineThymidineThe Journal of Neuroscience
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