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RESEARCH PRODUCT

Subventricular Zone Astrocytes Are Neural Stem Cells in the Adult Mammalian Brain

Arturo Alvarez-buyllaFiona DoetschIsabelle CailléDaniel A. LimJosé Manuel García-verdugo

subject

MaleRostral migratory streamanimal diseasesSubventricular zoneChick EmbryoBiologyGeneral Biochemistry Genetics and Molecular BiologyCerebral VentriclesSubgranular zoneMice03 medical and health sciences0302 clinical medicineNeuroblastNeurosphereGlial Fibrillary Acidic ProteinmedicineAnimalsRegeneration030304 developmental biology0303 health sciencesBiochemistry Genetics and Molecular Biology(all)Stem CellsBrainAnatomyOlfactory BulbNeural stem cell3. Good healthCell biologyNeuroepithelial cellmedicine.anatomical_structureNeuropoiesisnervous systemAstrocytes030217 neurology & neurosurgery

description

AbstractNeural stem cells reside in the subventricular zone (SVZ) of the adult mammalian brain. This germinal region, which continually generates new neurons destined for the olfactory bulb, is composed of four cell types: migrating neuroblasts, immature precursors, astrocytes, and ependymal cells. Here we show that SVZ astrocytes, and not ependymal cells, remain labeled with proliferation markers after long survivals in adult mice. After elimination of immature precursors and neuroblasts by an antimitotic treatment, SVZ astrocytes divide to generate immature precursors and neuroblasts. Furthermore, in untreated mice, SVZ astrocytes specifically infected with a retrovirus give rise to new neurons in the olfactory bulb. Finally, we show that SVZ astrocytes give rise to cells that grow into multipotent neurospheres in vitro. We conclude that SVZ astrocytes act as neural stem cells in both the normal and regenerating brain.

yearjournalcountryeditionlanguage
1999-06-01Cell
https://doi.org/10.1016/s0092-8674(00)80783-7