0000000000591030

AUTHOR

Sabina Schwenk-zieger

showing 3 related works from this author

Spheroid-based 3D Cell Cultures Enable Personalized Therapy Testing and Drug Discovery in Head and Neck Cancer.

2017

Background/Aim: Chemo-radiation currently serves as first-line therapy of advanced and recurrent head and neck cancer, while new chemotherapy regimens are emerging. However, response rates to any treatment are difficult to predict and underlie broad variation. This study shows the development of a standardized, high-throughput in vitro assay to assess patients' individual response to therapy regimens as a future tool for personalized tumor therapy. Materials and Methods: Viability and proliferation analyses after chemo +/- radiation treatment of single spheroids (low adhesion plates/Hanging Drop (HD)) were generated from head and neck squamous cell carcinoma (HNSCC) cell lines and primary h…

0301 basic medicineOncologyCancer Researchmedicine.medical_specialtyPathologyNecrosismedicine.medical_treatmentCell Culture TechniquesAntineoplastic Agents03 medical and health sciences3D cell culture0302 clinical medicineInternal medicineCell Line TumorSpheroids CellularDrug DiscoverymedicineTumor Cells CulturedHumansPrecision MedicineChemotherapybusiness.industryHead and neck cancerSpheroidGeneral MedicineChemoradiotherapymedicine.diseaseHead and neck squamous-cell carcinoma3. Good health030104 developmental biologyOncologyCell cultureHead and Neck Neoplasms030220 oncology & carcinogenesisPersonalized medicinemedicine.symptomDrug Screening Assays AntitumorbusinessAnticancer research
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Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma

2018

Current treatment options for advanced and recurrent head and neck squamous cell carcinoma (HNSCC) enclose radiation and chemo-radiation approaches with or without surgery. While platinum-based chemotherapy regimens currently represent the gold standard in terms of efficacy and are given in the vast majority of cases, new chemotherapy regimens, namely immunotherapy are emerging. However, the response rates and therapy resistance mechanisms for either chemo regimen are hard to predict and remain insufficiently understood. Broad variations of chemo and radiation resistance mechanisms are known to date. This study describes the development of a standardized, high-throughput in vitro assay to a…

0301 basic medicineOncologyCancer Researchmedicine.medical_specialtyGeneral Chemical Engineeringmedicine.medical_treatmentCell Culture TechniquesGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciences3D cell culture0302 clinical medicineInternal medicinemedicineCarcinomaHumansPrecision MedicineChemotherapyGeneral Immunology and MicrobiologySquamous Cell Carcinoma of Head and Neckbusiness.industryGeneral NeuroscienceHead and neck cancerImmunotherapymedicine.diseaseHead and neck squamous-cell carcinoma3. Good healthRegimen030104 developmental biologyHead and Neck Neoplasms030220 oncology & carcinogenesisCarcinoma Squamous CellPersonalized medicinebusinessJournal of Visualized Experiments
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Cytotoxic and genotoxic effects of electronic cigarette liquids on human mucosal tissue cultures of the oropharynx

2016

The popularity of electronic cigarettes (ECs) is rapidly growing and ECs are claimed to be an uncritically regarded alternative to conventional cigarettes. The mucosal tissue of the upper aerodigestive tract (UADT) is the first contact organ for xenobiotics such as liquids of ECs. The aim of this study is to investigate the bimolecular effects of e-liquids on human pharyngeal tissue cultures to evaluate whether e-liquids and their components present a risk factor for head and neck squamous cell carcinoma. Fresh tissue samples of healthy oropharyngeal mucosa were assembled into mucosal tissue cultures. Two fruit-flavored liquids (FLs), one tobacco-flavored liquid (TL) (all containing nicotin…

AdultMalePathologymedicine.medical_specialtyOropharyngeal TissueDNA damageCell SurvivalHealth Toxicology and MutagenesisOropharynxElectronic Nicotine Delivery SystemsToxicologyPathology and Forensic Medicine03 medical and health sciencesTissue cultureYoung Adult0302 clinical medicineFresh TissueRisk FactorsGermanyMedicineHumansCarcinogenMucous Membranebusiness.industryCytotoxinsSquamous Cell Carcinoma of Head and NeckGeneral Medicinemedicine.diseaseHead and neck squamous-cell carcinoma3. Good health030228 respiratory systemHead and Neck NeoplasmsToxicityCancer researchCarcinoma Squamous CellDNA fragmentationFemalebusiness030217 neurology & neurosurgeryDNA DamageMutagens
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