0000000000592990
AUTHOR
A. Benabdelmouna
Genome discrimination in progeny of interspecific hybrids between Brassica napus and Raphanus raphanistrum
International audience
Genomic in situ hybridization (GISH) discriminates between the A and the B genomes in diploid and tetraploid Setaria species
International audience
Southern and fluorescent in situ hybridization detect three RAPD-generated PCR products useful as introgression markers in Petunia
Fluorescent in situ hybridization (FISH) was used to reveal the intrachromosomal organization of 11 RAPD markers localized on the genetic map of Petunia hybrida. The cloned RAPD markers were analyzed by means of Southern hybridization to determine their level of sequence repetition and their specificity in different Petunia species with 2n=14 and 18 chromosomes. The same probes were then used in FISH experiments. Most of the RAPD clones studied showed high sequence repetition and no species specificity. Moreover, FISH analysis showed that these probes could belong to multilocus families as evidenced by the multiple FISH signals dispersed throughout the genome and present on every chromosome…
Copia-like retrotransposons in the genus Setaria : sequence heterogeneity, species distribution and chromosomal organization
International audience