The role of Odorant-Binding Proteins in nutrition under the control of microbiota

Desat expression in neural and non-neural tissues separately affects perception and emission of sex pheromones in Drosophila

The desaturase1 gene affects reproduction before, during and after copulation in Drosophila melanogaster

Desaturase1 (desat1) is one of the few genes known to be involved in the two complementary aspects of sensory communication — signal emission and signal reception — in Drosophila melanogaster. In p...

Function of odorant-binding proteins in the Drosophila melanogaster chemoreception

National audience; Function of odorant-binding proteins in the Drosophila [i]melanogaster[/i] chemoreception. 18. rencontre du Club de neurobiologie des invertébrés

JhI-21 is impacting glutamate receptor physiology at the larval neuromuscular junction in Drosophila

Poster

desat1 and the Evolution of Pheromonal Communication in Drosophila

1749-6632 (Electronic) 0077-8923; The evolution of communication is a fundamental biological problem. The genetic control of the signal and its reception must be tightly coadapted, especially in interindividual sexual communication. However, there is very little experimental evidence for tight genetic linkage connecting the emission of a signal and its reception. In Drosophila melanogaster, desat1 is the first known gene that simultaneously affects the emission and the perception of sex pheromones. Our experiments show that both aspects of pheromonal communication (the emission and the perception of sex pheromones) depend on distinct genetic control and may result from tissue-specific expre…

Dietary rescue of altered metabolism gene reveals unexpected [i]Drosophila[/i] mating cues

Running title: Dietary rescue of metabolic mutation and reproduction; To develop and reproduce, animals need long-chain Mono and PolyUnsaturated Fatty Acids (MUFAs; PUFAs). Although some UFAs can be synthesized by the organism, others must be provided by the diet. The gene desat1, involved in Drosophila melanogaster UFA metabolism, is necessary for both larval development and for adult sex pheromone communication. We first characterized desat1 expression in larval tissues. Then, we found that larvae in which desat1 expression was knocked down throughout development died during the larval stages when raised on standard food. By contrast pure MUFAs or PUFAs, but not saturated FAs, added to th…

Two sexually dimorphic Odorant binding proteins are affected by microbiota

Tenectin, a novel extracellular matrix protein expressed during Drosophila melanogaster embryonic development

1567-133X (Print) Journal Article Research Support, Non-U.S. Gov't; During Drosophila embryonic development, various morphogenetic processes require the remodeling of the extracellular matrix. In a previous study, we have identified and characterized a cDNA encoding a novel putative extracellular matrix protein named tenebrin, in the beetle Tenebrio molitor. Here, we examine the expression of the Drosophila ortholog, referred to as Tenectin (Tnc), during embryonic development. Tnc is expressed in the majority of tissues of neuroectodermic origin such as hindgut, foregut, tracheal system, anal plate, and CNS. In the CNS, the Tnc transcript is restricted to a few cells, whereas the protein is…

Tenectin is a novel alphaPS2betaPS integrin ligand required for wing morphogenesis and male genital looping in Drosophila.

International audience; Morphogenesis of the adult structures of holometabolous insects is regulated by ecdysteroids and juvenile hormones and involves cell-cell interactions mediated in part by the cell surface integrin receptors and their extracellular matrix (ECM) ligands. These adhesion molecules and their regulation by hormones are not well characterized. We describe the gene structure of a newly described ECM molecule, tenectin, and demonstrate that it is a hormonally regulated ECM protein required for proper morphogenesis of the adult wing and male genitalia. Tenectin's function as a new ligand of the PS2 integrins is demonstrated by both genetic interactions in the fly and by cell s…

Implication de l’OBP19b dans la perception gustative des acides aminés chez Drosophila melanogaster

National audience; La chimioperception est nécessaire à la détection des aliments nutritifs et à l’identification des composés toxiques, d’une niche écologique appropriée et des partenaires sexuels. Comme beaucoup d'autres insectes, la mouche du vinaigre, Drosophila melanogaster, possède des capacités chimiosensorielles performantes, la rendant très sensible en lui permettant de détecter et discriminer un large panel de substances chimiques. Chez la Drosophile, cette détection est réalisée par les systèmes chimiosensoriels gustatifs et olfactifs impliquant plusieurs familles de récepteurs. Les composés chimiques volatils et non volatils qui pénétrent les organes sensoriels (sensilles) doive…

OBP19b is involved in the taste perception of amino acid in Drosophila melanogaster

Chemoperception plays a crucial role in the detection of nutritive food and avoidance of toxic compounds. It also allows animals to identify a suitable ecological niche and mating partners. As many other insects, Drosophila melanogaster possess a very sensitive chemosensory ability to detect and discriminate a wide panel of semiochemicals. Chemosensory detection is mostly mediated by olfactory and gustatory systems involving several multigene chemoreceptor families. Volatile and non-volatile chemicals compounds entering the sensory organ (sensillum) must be solubilized before being transported through the hydrophilic sensillum lymph bathing chemosensory receptors. Such “perireceptor events”…

The desaturase1 gene affects reproduction before, during and after copulation in Drosophila melanogaster

Desaturase1 (desat1) is one of the few genes known to be involved in the two complementary aspects of sensory communication — signal emission and signal reception — in Drosophila melanogaster. In particular, desat1 is necessary for the biosynthesis of major cuticular pheromones in both males and females. It is also involved in the male ability to discriminate sex pheromones. Each of these two sensory communication aspects depends on distinct desat1 putative regulatory regions. Here, we used (i) mutant alleles resulting from the insertion/excision of a transposable genomic element inserted in a desat1 regulatory region, and (ii) transgenics made with desat1 regulatory regions used to target …